Document 0841
 DOCN  M9540841
 TI    Mycoplasma stimulates HIV-1 expression from acutely- and
       dormantly-infected promonocyte/monoblastoid cell lines.
 DT    9504
 AU    Chowdhury MI; Munakata T; Koyanagi Y; Arai S; Yamamoto N; Department of
       Microbiology, Tokyo Medical and Dental University; School of Medicine,
       Japan.
 SO    Arch Virol. 1994;139(3-4):431-8. Unique Identifier : AIDSLINE
       MED/95134098
 AB    Treatment of a myelo-monocyte cell line, J22HL-60, dormantly infected
       with human immunodeficiency virus type 1 (HIV-1) with heat-inactivated
       extracts of Acholeplasma (A) laidlawii (250 micrograms/ml) enhanced
       virus production more than 45-fold as assessed by p24 viral core antigen
       assay. When treated with a suboptimal dose of TPA or TNF-alpha,
       Acholeplasma extracts further augmented virus production in J22HL-60
       cells. H7, an inhibitor of protein kinase C(PKC), almost completely
       abrogated HIV-1-inducing ability of Acholeplasma extracts in the cells.
       A. laidlawii and several other mycoplasmas also enhanced acute infection
       of U937 cells as shown by increased virus-positive cells and
       augmentation of HIV-1 production in the culture supernatant independent
       of their pathogenicity to humans.
 DE    Acholeplasma laidlawii/*PHYSIOLOGY  Cell Line  Human  HIV Core Protein
       p24/ANALYSIS  HIV-1/*PHYSIOLOGY  Isoquinolines/PHARMACOLOGY
       Macrophages/*VIROLOGY  Monocytes/*VIROLOGY  Mycoplasma/*PHYSIOLOGY
       Piperazines/PHARMACOLOGY  Protein Kinase C/ANTAGONISTS & INHIB  Support,
       Non-U.S. Gov't  Tetradecanoylphorbol Acetate/PHARMACOLOGY  Tumor Cells,
       Cultured  Tumor Necrosis Factor/PHARMACOLOGY  Virus Replication  JOURNAL
       ARTICLE

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