Document 0858
 DOCN  M9540858
 TI    Physical contact with lymphocytes is required for reactivation of
       dormant HIV-1 in colonic epithelial cells: involvement of the HIV-1 LTR.
 DT    9504
 AU    Faure E; Yahi N; Zider A; Cavard C; Champion S; Fantini J; Institut de
       Chimie Biologique, Universite de Provence,; Marseille, France.
 SO    Virus Res. 1994 Oct;34(1):1-13. Unique Identifier : AIDSLINE
       MED/95133350
 AB    HIV-1 transmission from mucosal epithelial cells to lymphocytes is a
       potential mechanism of HIV-1 contamination during sexual intercourse.
       The human colon epithelial cell line HT-29, that is infectable by
       various HIV-1 strains, is a useful model for studying the molecular
       mechanisms involved in this process. In the present study, we show that
       HT-29 cells, when exposed to either HIV-1(LAI) or HIV-1(NDK) at a low
       multiplicity of infection, became infected but did not produce
       infectious virions. Using two-compartment cell culture chambers
       separated by a porous membrane, we showed that PBL were able to rescue
       infectious HIV-1 from latently infected HT-29 cells following a physical
       interaction between the two cell populations. In contrast, HT-29 cells,
       infected with the same viruses at a high multiplicity of infection, were
       able to produce mature viral particles that were infectious to PBL in
       absence of cellular contacts. Transient expression assays using an
       indicator gene under the control of the HIV-1 long terminal repeat
       revealed that cell-to-cell contact induced an activation of the HIV-1
       promoter. These observations provide a putative molecular mechanism for
       transmission of HIV-1 from mucosal epithelial cells to lymphocytes.
 DE    *Cell Communication  Cell Line  Cell Line, Transformed  Cereals/CYTOLOGY
       Colon/CYTOLOGY/VIROLOGY  Comparative Study  Culture Media,
       Conditioned/PHARMACOLOGY  Epithelium/CYTOLOGY/VIROLOGY  *Gene Expression
       Regulation, Viral  Genes, Reporter  Genes, Synthetic  Human  *HIV Long
       Terminal Repeat  HIV-1/*PHYSIOLOGY  Intestinal Mucosa/CYTOLOGY/*VIROLOGY
       Lymphokines/PHARMACOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes/*CYTOLOGY  *Virus Activation  Virus Replication  JOURNAL
       ARTICLE

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