Document 0862
 DOCN  M9540862
 TI    Full expression of transfected nonproducer interfering HIV-1 proviral
       DNA abrogates susceptibility of human He-La CD4+ cells to HIV.
 DT    9504
 AU    Federico M; Nappi F; Bona R; D'Aloja P; Verani P; Rossi GB; Laboratory
       of Virology, Istituto Superiore di Sanita, Rome,; Italy.
 SO    Virology. 1995 Jan 10;206(1):76-84. Unique Identifier : AIDSLINE
       MED/95133225
 AB    An Hut-78 cell clone (F12) harboring a nonproducer human
       immunodeficiency virus-1 (HIV) variant, and showing a complete
       resistance to HIV-1 or HIV-2 superinfection, was previously
       characterized. We demonstrated that the replication of the
       superinfecting HIVs is blocked at the retrotranscription step, despite
       the CD4 down-regulation, since HIVs are able to cross the Hut-78/F12
       cell membrane. In order to establish if the expression of the HIV-1
       variant (F12/HIV) could be per se sufficient to induce the homologous
       viral interference shown in the F12 cells, the whole F12/HIV provirus
       was cloned and transfected in He-La CD4+ cells. In F12/HIV expressing
       He-La CD4+ clones, both the viral proteins expressed and the HIV
       nonproducer phenotype remain unmodified compared to F12 cells.
       Furthermore, despite the full expression of CD4 HIV receptors, the life
       cycle of the superinfecting HIV could be either strongly inhibited or
       totally abolished, depending on the cell clone considered. The
       inhibition of the superinfecting HIV was also reproduced when an HIV
       infectious molecular clone was transfected in F12/HIV He-La CD4+ clones,
       thus indicating that a post-cDNA synthesis block may operate against the
       superinfecting HIV. These data demonstrate that HIV susceptibility could
       be abrogated in cells expressing the F12/HIV genome, even in absence of
       any CD4 down-regulation.
 DE    Antigens, CD4/GENETICS  Cloning, Molecular  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY/*VIROLOGY  DNA, Viral/*BIOSYNTHESIS  Hela Cells
       Human  HIV-1/*GENETICS/PATHOGENICITY  Plasmids  Proviruses/*GENETICS
       Support, Non-U.S. Gov't  Transfection  Viral Interference  JOURNAL
       ARTICLE

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