Document 0867
 DOCN  M9540867
 TI    Multiple determinants for growth of human immunodeficiency virus type 1
       in monocyte-macrophages.
 DT    9504
 AU    Malykh A; Reitz MS Jr; Louie A; Hall L; Lori F; Laboratory of Tumor Cell
       Biology, National Cancer Institute,; National Institutes of Health,
       Bethesda, Maryland 20892.
 SO    Virology. 1995 Jan 10;206(1):646-50. Unique Identifier : AIDSLINE
       MED/95133203
 AB    Attempts to define the genetic determinants required for efficient
       growth of human immunodeficiency virus type 1 (HIV-1) in
       monocyte-macrophages were made by constructing chimeras between two
       infectious clones of HIV-1 (HXB2 and LW/C), which despite only minor
       differences in their DNA sequence have striking differences in cell
       tropism. Although both of them replicate efficiently in peripheral blood
       mononuclear cells, HXB2 replicates extensively in permanent T cell lines
       but poorly in primary monocyte macrophages (T cell line tropic); the
       reverse is true for LW/C (macrophage tropic). The envelope proved to
       contain the major determinants of macrophage tropism. However, tropism
       determinants appeared to be scattered along the envelope. In particular,
       the V3 loop alone appeared to be neither necessary nor sufficient for
       growth in macrophages. Both vpr and nef genes appeared to play a less
       significant role to improve viral replication in macrophages, but only
       in the presence of the proper envelope sequences. HIV-1 macrophage
       tropism thus appears to result from the contribution of several
       different determinants.
 DE    Amino Acid Sequence  Base Sequence  Cell Line  DNA Primers  Genes, Viral
       Human  HIV-1/*PHYSIOLOGY  Macrophages/*VIROLOGY  Molecular Sequence Data
       Monocytes/*VIROLOGY  *Virus Replication/GENETICS  JOURNAL ARTICLE

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