Document 0868
 DOCN  M9540868
 TI    Restricted replication of the HIV-1 T-lymphotropic isolate NL4-3 in
       HL-60 cells.
 DT    9504
 AU    Pise-Masison CA; Holland CA; Department of Molecular Genetics and
       Microbiology, University of; Massachusetts Medical Center, Worcester.
 SO    Virology. 1995 Jan 10;206(1):641-5. Unique Identifier : AIDSLINE
       MED/95133202
 AB    To understand how different cell types might influence the generation of
       viral variants, we have examined the differences in the viral life cycle
       of the HIV-1 isolate, NL4-3, in the human promyelocytic cell line,
       HL-60, and the human T cell line, H9. NL4-3 harvested from H9 cells
       productively infected and was cytopathic to H9 and HL-60 cells. However,
       the cytopathic effect was delayed in HL-60 cells compared to that seen
       in H9 cells, suggesting that NL4-3 replication was restricted in myeloid
       cells. This restriction was overcome by production of a variant virus,
       NL4-3 (M), which replicated efficiently in HL-60 cells. Measurements of
       the kinetics of entry of NL4-3 in H9 and HL-60 cells and NL4-3 (M) in
       HL-60 cells demonstrated that the timing of viral entry into each cell
       line was similar. However, quantitation of the amount of newly
       reverse-transcribed NL4-3 DNA in H9 and HL-60 cells revealed that
       NL4-3-infected H9 cells and NL4-3 (M)-infected HL-60 cells contained
       consistently more newly reverse-transcribed DNA than NL4-3-infected
       HL-60 cells. This difference was further amplified by inefficient spread
       of the virus throughout the HL-60 culture. Together these results
       suggest that the efficiency of NL4-3 infection of HL-60 cells is
       restricted at early steps in the viral life cycle and may be restricted
       at late steps as well.
 DE    Base Sequence  Cell Line  Cytopathogenic Effect, Viral  DNA Primers
       DNA, Viral/ANALYSIS  Human  HIV-1/*PHYSIOLOGY  Membrane Fusion
       Molecular Sequence Data  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*VIROLOGY  *Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).