Document 0869
 DOCN  M9540869
 TI    Establishment of leukemic T-cell lines from mice inoculated with the
       MAIDS defective virus.
 DT    9504
 AU    Simard C; Huang M; Jolicoeur P; Laboratory of Molecular Biology,
       Clinical Research Institute of; Montreal, Quebec, Canada.
 SO    Virology. 1995 Jan 10;206(1):555-63. Unique Identifier : AIDSLINE
       MED/95133192
 AB    Mice inoculated with replication-competent stocks of the murine acquired
       immunodeficiency syndrome (MAIDS) virus are severely immunocompromised
       and proned to the development of T- and B-cell lymphomas. We have
       studied the development of T-cell lymphomas in C57BL/6 and RF/J mice
       inoculated with helper-free stocks of the MAIDS defective virus. We
       observed the expansion of T cell clones (detected by TCR gene
       rearrangements and by transplantation) only rarely in diseased C57BL/6
       mice and slightly more frequently in RF/J mice. We succeeded in
       establishing four transplantable T cell tumors and malignant cell lines.
       The three cell lines from RF/J mice were immature T-cells (Thy-1+, CD3-,
       CD4+, CD8+, Mac-1+), while the line from the C57BL/6 mouse had the
       phenotype of mature T-cells (Thy-1+, CD3+, CD4+, CD8-). All lines were
       virus-producers despite the fact that helper-free stocks of the virus
       were inoculated. These helper MuLVs most likely originated from
       endogenous MuLV sequences. Also, the defective viral genome was clearly
       detectable in one cell line and was rearranged in two other lines. These
       established cell lines may be useful to determine whether they share
       some of the characteristics of the anergic T-cells in vivo and to study
       the role of the MAIDS defective virus in T cell transformation.
 DE    Animal  Cell Membrane/IMMUNOLOGY  *Cell Transformation, Neoplastic
       *Cell Transformation, Viral  Leukemia Viruses, Murine/*PHYSIOLOGY
       Leukemia, T-Cell/IMMUNOLOGY/*VIROLOGY  Lymphoma, T-Cell/VIROLOGY  Mice
       Mice, Inbred C57BL  Murine Acquired Immunodeficiency
       Syndrome/*IMMUNOLOGY/VIROLOGY  Neoplasm Transplantation  Phenotype
       Support, Non-U.S. Gov't  Tumor Cells, Cultured  JOURNAL ARTICLE

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