Document 0876
 DOCN  M9540876
 TI    Enhancement of HIV-1 proteinase activity by HIV-1 reverse transcriptase.
 DT    9504
 AU    Goobar-Larsson L; Luukkonen BG; Unge T; Schwartz S; Utter G; Strandberg
       B; Oberg B; Microbiology and Tumorbiology Center (MTC), Karolinska
       Institute,; Stockholm, Sweden.
 SO    Virology. 1995 Jan 10;206(1):387-94. Unique Identifier : AIDSLINE
       MED/95133173
 AB    HIV-1 reverse transcriptase (RT) was found to increase the activity of
       HIV-1 proteinase in vitro and in eukaryotic cells. The effect of RT on
       proteinase activity was dose-dependent and independent of pH or salt
       concentration. The cleavage of sequences corresponding to all the
       naturally occurring cleavage sites that could be tested in vitro was
       enhanced. The effect of RT on cleavage was greatest at the cleavage site
       between RT and integrase. The enhancement of viral proteinase activity
       by the virus RT may contribute to regulation of the order and/or
       efficiency of cleavage at different sites during virus replication and
       maturation.
 DE    Base Sequence  Enzyme Activation  Hela Cells  Human  Hydrogen-Ion
       Concentration  HIV Protease/*METABOLISM  HIV-1/*ENZYMOLOGY  Molecular
       Sequence Data  Oligodeoxyribonucleotides  Osmolar Concentration  Reverse
       Transcriptase/*METABOLISM  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).