Document 0887
 DOCN  M9540887
 TI    Induction of antibodies to the human immunodeficiency virus type 1 by
       immunization of baboons with immunoglobulin molecules carrying the
       principal neutralizing determinant of the envelope protein.
 DT    9504
 AU    Zaghouani H; Anderson SA; Sperber KE; Daian C; Kennedy RC; Mayer L; Bona
       CA; Department of Microbiology, Mount Sinai School of Medicine, New;
       York, NY 10029.
 SO    Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):631-5. Unique Identifier :
       AIDSLINE MED/95132652
 AB    The hypervariable region 3 (V3) within the disulfide-bridged loop of the
       envelope protein of the human immunodeficiency virus type 1 (HIV-1)
       contains an amino acid sequence that was defined as a principal
       neutralizing determinant (PND). A 19-amino acid residue consensus
       sequence (designated V3C) predicted from the PND sequences of 245
       isolates as well as a sequence from the PND of the WMJ2 HIV-1 isolate
       (designated V3M) were expressed on the variable region of murine-human
       immunoglobulin (Ig) chimeras that were designated Ig-V3C and Ig-V3M,
       respectively. The HIV-1 sequences on the Ig chimeras preserved their
       antigenicity and interacted with antibodies specific for peptides
       encompassing the V3C and V3M sequences. In baboons, Ig-V3C and Ig-V3M
       induced antibodies that bound V3C and V3M peptides as well as the
       glycoprotein gp120 envelope protein of HIV-1 MN isolate. In addition,
       the baboons' antisera were able to prevent infection of CD4 SupT1
       susceptible T cells by HIV-1 MN. Finally, Ig-V3M chimeras were able to
       stimulate in vitro production of antibodies specific for the HIV-1
       envelope-derived peptides by lymphocytes from HIV-1-infected human
       subjects.
 DE    Amino Acid Sequence  Animal  AIDS Vaccines/GENETICS/*IMMUNOLOGY
       Chimeric Proteins/IMMUNOLOGY  Human  HIV Antibodies/BIOSYNTHESIS  HIV
       Envelope Protein gp120/GENETICS/IMMUNOLOGY  HIV Infections/*PREVENTION &
       CONTROL  HIV-1/GENETICS/*IMMUNOLOGY  *Immunization  Immunodominant
       Epitopes/GENETICS/IMMUNOLOGY  Immunoglobulins/GENETICS/IMMUNOLOGY  Male
       Mice  Molecular Sequence Data  Papio/IMMUNOLOGY  Peptide
       Fragments/GENETICS/IMMUNOLOGY  Protein Engineering  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  Vaccines,
       Synthetic/GENETICS/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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