Document 0925
 DOCN  M9540925
 TI    Local immune responses in the rat cerebral cortex after middle cerebral
       artery occlusion.
 DT    9504
 AU    Schroeter M; Jander S; Witte OW; Stoll G; Department of Neurology,
       Heinrich-Heine-Universitat,; Dusseldorf, Germany.
 SO    J Neuroimmunol. 1994 Dec;55(2):195-203. Unique Identifier : AIDSLINE
       MED/95130728
 AB    This study describes local immune responses in cerebral ischemia induced
       by permanent occlusion of the middle cerebral artery (MCAO) in the rat.
       The temporal and spatial pattern of leukocyte infiltration was
       characterized immunocytochemically using monoclonal antibodies against
       CD5, a pan T cell marker, against CD4 and CD8 for subtyping of T
       lymphocytes, and ED1, a marker for macrophages. CD5+ T cells were
       present in some animals on the pial surface at day 1 and with increasing
       numbers mainly at the edges of the infarcts at days 3 and 7. By day 14
       their number had significantly decreased. Subtyping of T lymphocytes
       revealed that CD4+ helper/inducer T cells were rare, while CD8+
       lymphocytes were abundant. Moreover, CD8+ lymphocytes outnumbered CD5+ T
       cells indicating the presence of CD5-/CD8+ natural killer (NK) cells.
       ED1+ macrophages primarily infiltrated the core of the infarct starting
       on day 1. Infiltrating leukocytes expressed leukocyte function
       associated antigen-1 and MHC class I and II antigens. Early after
       infarction, increased expression of the intercellular adhesion
       molecule-1 was found on vessels and leukocytes. In conclusion, this
       study shows that lymphocytes enter the nervous system not only in
       autoimmune diseases, but also in response to primarily 'non-immune'
       neuronal damage such as stroke.
 DE    Animal  Antigens, CD/ANALYSIS  Cerebral Arteries  Cerebral Cortex/BLOOD
       SUPPLY/*IMMUNOLOGY  Cerebral Ischemia/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Intercellular Adhesion Molecule-1/ANALYSIS
       Killer Cells, Natural/IMMUNOLOGY  Male  Rats  Rats, Inbred Lew  Rats,
       Wistar  Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY  JOURNAL
       ARTICLE

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