Document 0928
 DOCN  M9540928
 TI    Enhanced diagnostic efficiency of the polymerase chain reaction by
       co-amplification of multiple regions of HIV-1 and HIV-2.
 DT    9504
 AU    Udaykumar; Heredia A; Soriano V; Bravo R; Epstein JS; Hewlett IK;
       Laboratory of Molecular Virology, Food and Drug Administration,;
       Rockville, MD 20852-1448.
 SO    J Virol Methods. 1994 Aug;49(1):37-46. Unique Identifier : AIDSLINE
       MED/95130642
 AB    A method for co-amplification of multiple viral sequences of HIV-1 and
       HIV-2 by polymerase chain reaction was designed. The technique resulted
       in the specific detection of each type of virus and allowed the
       amplification of as few as two copies of target DNA. The amplification
       of multiple regions of the viral genome offers the advantage of
       detecting multiple target sequences, which may be essential for some
       viruses, such as HIV, that exhibit a high degree of variability in their
       gene sequences. In addition, the method permitted the detection of both
       virus types in the same reaction, allowing for greater utility in
       geographic areas where coinfections with both viruses occur and
       cross-reactivity in Western blots is observed. This method was applied
       successfully to the detection of viral DNA in clinical specimens.
 DE    Base Sequence  DNA Primers/GENETICS  DNA Probes/GENETICS  DNA,
       Viral/GENETICS  Gene Amplification  Genes, env  Genes, gag  Genes, pol
       Human  HIV Infections/*DIAGNOSIS/VIROLOGY  HIV-1/*GENETICS
       HIV-2/*GENETICS  Molecular Sequence Data  Polymerase Chain
       Reaction/*METHODS/STATISTICS & NUMER DATA  Sensitivity and Specificity
       Virology/METHODS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).