Document 0929
 DOCN  M9540929
 TI    Reference preparations in the standardisation of HIV-1 PCR--an
       international collaborative study.
 DT    9504
 AU    Bootman JS; Kitchin PA; PCR Reference Centre, AIDS Collaborating Centre,
       NIBSC, Potters; Bar, Herts, UK.
 SO    J Virol Methods. 1994 Aug;49(1):1-8. Unique Identifier : AIDSLINE
       MED/95130640
 AB    Following the recent developments in diagnostic polymerase chain
       reaction (PCR) technology, we have assessed a set of HIV-1 DNA reference
       standards using the first commercial diagnostic test kit for the
       detection of HIV-1 (Amplicor, Roche) in an international collaborative
       study. Nineteen laboratories in 11 countries analysed a set of ten
       (re-coded) HIV-1 DNA reference standards, whose performance had been
       validated in a previous collaborative study (Bootman and Kitchin, 1992).
       Results from the current study show that, using the diagnostic kit, 84%
       of laboratories (16/19) obtained correct diagnoses for all ten test
       samples. One additional laboratory failed only to detect the sample
       containing ten copies of target template. Test results from the
       remaining two laboratories were declared void in accordance with the
       Amplicor quality control guidelines.
 DE    DNA, Viral/GENETICS/STANDARDS  False Negative Reactions  False Positive
       Reactions  Human  HIV Infections/DIAGNOSIS  HIV-1/*GENETICS
       International Cooperation  Polymerase Chain Reaction/METHODS/STATISTICS
       & NUMER DATA/  *STANDARDS  Quality Control  Reference Standards
       Reproducibility of Results  Sensitivity and Specificity
       Virology/METHODS/STATISTICS & NUMER DATA/STANDARDS  JOURNAL ARTICLE

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