Document 0938
 DOCN  M9540938
 TI    Vaccination of rabbits with recombinant vaccinia virus carrying the
       envelope gene of human T-cell lymphotropic virus type I.
 DT    9504
 AU    Hakoda E; Machida H; Tanaka Y; Morishita N; Sawada T; Shida H; Hoshino
       H; Miyoshi I; Department of Medicine, Kochi Medical School, Japan.
 SO    Int J Cancer. 1995 Feb 8;60(4):567-70. Unique Identifier : AIDSLINE
       MED/95130294
 AB    Two groups of 3 rabbits each were immunized with either recombinant
       vaccinia virus, WR-SFB5env, carrying the human T-cell lymphotropic virus
       type I (HTLV-I) env gene at the site of the hemagglutinin gene of the WR
       strain, or control vaccinia virus, HA-WR, lacking the functional
       hemagglutinin gene. All 6 rabbits responded with anti-vaccinia virus
       antibodies. WR-SFB5env elicited anti-HTLV-I env antibodies but no
       vesicular stomatitis virus (HTLV-I) pseudotype neutralizing antibodies
       in all 3 rabbits. After 10 weeks, the animals were challenged by
       transfusion of blood from an HTLV-I-infected rabbit. Two of the 3
       vaccinated rabbits and all 3 control rabbits became infected with
       HTLV-I, as indicated by seroconversion and detection of HTLV-I proviral
       sequences by polymerase chain reaction. The rabbit that had been
       protected from initial challenge became infected with HTLV-I upon
       rechallenge 12 weeks after the first challenge. In view of the proven
       prophylactic effect of passive immunization against HTLV-I, our vaccine
       trial failed because WR-SFB5env was incapable of inducing neutralizing
       antibodies against HTLV-I in the immunized animals. It remains to be
       studied whether cell-mediated immunity such as antibody-dependent
       cellular cytotoxicity was involved in the temporary protection of I
       vaccinated rabbit.
 DE    Animal  Blotting, Western  Drug Screening  Genes, env/IMMUNOLOGY
       HTLV-I/*GENETICS/IMMUNOLOGY  HTLV-I Antibodies/*BIOSYNTHESIS/BLOOD
       HTLV-I Infections/*PREVENTION & CONTROL  Neutralization Tests
       Polymerase Chain Reaction  Rabbits  Support, Non-U.S. Gov't  *Vaccines,
       Synthetic  Vaccinia Virus/IMMUNOLOGY  Vesicular Stomatitis-Indiana
       Virus/IMMUNOLOGY  *Viral Vaccines  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).