Document 0951
 DOCN  M9540951
 TI    A new phage display system to construct multicombinatorial libraries of
       very large antibody repertoires.
 DT    9504
 AU    Geoffroy F; Sodoyer R; Aujame L; Department of Molecular Immunology,
       Pasteur Merieux Serums et; Vaccins, Marcy l'Etoile, France.
 SO    Gene. 1994 Dec 30;151(1-2):109-13. Unique Identifier : AIDSLINE
       MED/95129840
 AB    We present an easy and efficient technique for the construction of large
       phage-displayed antibody (Ab) repertoires through the recombination of
       two separate heavy (VH) and light (VL) chain gene libraries. Here, the
       system has been applied to the display of a chimpanzee anti-HIV gp160
       Ab. The process, which makes use of lambda phage att recombination
       sites, leads to the irreversible physical association between plasmid
       and phagemid vectors carrying, respectively, VL and VH sequences. The
       heat-inducible expression of the Int recombinase allows perfect control
       of recombination. Selection of the recombinant phagemid is made possible
       by the assembly, in vivo, of a genetic marker (chloramphenicol
       resistance) created only after the correct recombination event.
       Theoretically, all possible associations between the VL and VH sequences
       should be obtained, and it should be possible to generate
       multicombinatorial libraries of close to 10(12) clones.
 DE    Animal  Chimpansee troglodytes  Enzyme-Linked Immunosorbent Assay  *Gene
       Library  Gene Products, env/ANALYSIS/*IMMUNOLOGY  *Genes, Immunoglobulin
       HIV/IMMUNOLOGY/ISOLATION & PURIF  HIV Antibodies/*GENETICS
       Immunoglobulins, Fab/ANALYSIS/BIOSYNTHESIS/GENETICS  Immunoglobulins,
       Heavy-Chain/*GENETICS  Immunoglobulins, Light-Chain/*GENETICS  Plasmids
       Protein Precursors/ANALYSIS/*IMMUNOLOGY  Recombination, Genetic
       Restriction Mapping  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).