Document 0961
 DOCN  M9540961
 TI    Repair of immunoglobulin response in B cell line (JK32.1) originating
       from immunodeficient patient via implantation of functional plasma
       membranes.
 DT    9504
 AU    Ben-Anat Porat Y; Zan-Bar I; Department of Human Microbiology, Sackler
       School of Medicine,; Tel-Aviv University, Israel.
 SO    Clin Immunol Immunopathol. 1995 Feb;74(2):151-5. Unique Identifier :
       AIDSLINE MED/95129291
 AB    Human-human B cell hybridoma JK32.1, constructed from B lymphocytes of a
       common variable immunodeficient patient and nonsecreting cell line,
       retains the defects of B cell immunodeficiency. Efforts to clarify
       whether the defect is located within the plasma membranes of this cell
       line were carried out by implanting them with plasma membrane fraction
       derived from normal functional cells via intact non-infectious Sendai
       virus. The implanted cells were activated with various mitogens and
       their Ig responses and isotype switching were examined. Restoration of
       IgM secretion was achieved in the implanted JK32.1 cells following
       stimulation with SAC, PWM, or retinoic acid. Augmented IgM response was
       also obtained in the implanted cells treated with retinoic acid and
       lipopolysaccharide (LPS) despite their unresponsiveness to LPS alone. No
       IgG or IgA response could be detected in the implanted JK32.1 cells.
       These data suggest that this immunodeficient cell line possesses at
       least two different malfunctions, one located within the plasma membrane
       moiety of the cells and the other located within the cytoplasmic and/or
       nucleic components. The plasma membrane moiety defect can be repaired
       temporarily by delivering proper signals via the implanted plasma
       membranes. However, this manipulation of the cells could not overcome
       the intrinsic defect of the cells which blocks isotype switching and
       secretion of IgG, IgE, and IgA antibodies.
 DE    Animal  B-Lymphocytes/*IMMUNOLOGY/*ULTRASTRUCTURE  Cell Line  Cell
       Membrane/*IMMUNOLOGY  Common Variable Immunodeficiency/*IMMUNOLOGY
       Female  Human  IgM/*BIOSYNTHESIS  Lipopolysaccharides/IMMUNOLOGY
       Membrane Fusion/*IMMUNOLOGY  Mice  Mice, Inbred BALB C
       Mitogens/IMMUNOLOGY  Phytohemagglutinins/IMMUNOLOGY  Pokeweed
       Mitogens/IMMUNOLOGY  Staphylococcus aureus/IMMUNOLOGY  Support, Non-U.S.
       Gov't  Tretinoin/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).