Document 0965
 DOCN  M9540965
 TI    CD4+ T lymphocytes infiltrating human breast cancer recognise autologous
       tumor in an MHC-class-II restricted fashion.
 DT    9504
 AU    Dadmarz R; Sgagias MK; Rosenberg SA; Schwartzentruber DJ; Surgery
       Branch, National Cancer Institute, National Institutes of; Health,
       Bethesda, MD 20892-1502.
 SO    Cancer Immunol Immunother. 1995 Jan;40(1):1-9. Unique Identifier :
       AIDSLINE MED/95129071
 AB    Tumor-infiltrating lymphocytes (TIL) were derived from primary breast
       tumors, metastatic lymph nodes and malignant pleural effusions from 34
       patients with breast cancer. TIL were cultured for approximately 30 days
       and studied for phenotype, cytotoxicity, and the ability to secrete
       cytokines in response to autologous tumor stimulation. Tumor specimens
       were obtained from two different sites in 7 patients, resulting in 41
       samples from which 38 TIL cultures were established. In addition to
       screening 38 bulk TIL cultures, TIL from 21 patients were separated into
       CD4+ and CD8+ subsets and extensively studied. Three CD4+ TIL were found
       specifically to secrete granulocyte macrophage-colony-stimulating factor
       and tumor necrosis factor alpha when stimulated by autologous tumor and
       not by a large panel of stimulators (24-34) consisting of autologous
       normal cells, allogeneic breast or melanoma tumors and EBV-B cells. This
       cytokine release was found to be MHC-class-II-restricted, as it was
       inhibited by the anti-HLA-DR antibody L243. These 3 patients' EBV-B
       cells, when pulsed with tumor lysates, were unable to act as
       antigen-presenting cells and induce cytokine secretion by their
       respective CD4+ TIL. These findings demonstrate that
       MHC-class-II-restricted CD4+ T cells recognising tumor-associated
       antigens can be detected in some breast cancer patients.
 DE    Adult  Antibodies, Monoclonal  Breast Neoplasms/*IMMUNOLOGY  Cell Line,
       Transformed  Cytokines/SECRETION  Cytotoxicity, Immunologic
       CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Female  Histocompatibility Antigens Class
       II/*IMMUNOLOGY  Human  Lymphocytes, Tumor-Infiltrating/*IMMUNOLOGY
       Tumor Cells, Cultured  JOURNAL ARTICLE

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