Document 0981
 DOCN  M9540981
 TI    Oligosaccharide profiles of HIV-2 external envelope glycoprotein:
       dependence on host cells and virus isolates.
 DT    9504
 AU    Liedtke S; Adamski M; Geyer R; Pfutzner A; Rubsamen-Waigmann H; Geyer H;
       Biochemisches Institut am Klinikum der Universitat, Giessen,; FRG.
 SO    Glycobiology. 1994 Aug;4(4):477-84. Unique Identifier : AIDSLINE
       MED/95128086
 AB    The glycosylation pattern of the external envelope glycoprotein of human
       immunodeficiency virus type 2 (HIV-2) was studied in dependence on host
       cells and virus isolates. Strains HIV-2ALT, HIV-2ROD and HIV-2D194,
       differing in their biological properties and in the amino acid sequences
       of their env genes, were propagated in MOLT4, HUT78 and U937 cells, in
       human peripheral blood lymphocytes and monocytes/macrophages in the
       presence of [6-3H]glucosamine. Radiolabelled viral glycoproteins were
       isolated from the cell-free supernatants and digested with trypsin.
       Glycans were sequentially liberated by endo-beta-N-acetylglucosaminidase
       H and peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase F, and
       fractionated according to charge and size. Comparison of the
       oligosaccharide profiles revealed that the envelope glycoproteins of
       different virus isolates, propagated in the same host cells, yielded
       very similar glycan patterns, whereas cultivation of an isolate in
       different host cells resulted in markedly divergent oligosaccharide
       maps. Variations concerned the proportion of high-mannose-, hybrid- and
       complex-type substituents, as well as the state of charge and structural
       parameters of the complex-type species. As a characteristic feature,
       complex-type glycans of macrophage-derived viral glycoprotein were
       almost exclusively substituted by lactosamine repeats. Hence,
       glycosylation of the HIV-2 external envelope glycoprotein seems to be
       primarily governed by host cell-specific factors rather than by the
       amino acid sequence of the corresponding polypeptide backbone.
 DE    Carbohydrate Sequence  Cell Line  Comparative Study  Electrochemistry
       Gene Products, env/*CHEMISTRY/ISOLATION & PURIF  Human  HIV
       Infections/VIROLOGY  HIV-2/*CHEMISTRY/ISOLATION & PURIF
       Leukocytes/VIROLOGY  Molecular Sequence Data
       Oligosaccharides/*CHEMISTRY  Polysaccharides/CHEMISTRY  Support,
       Non-U.S. Gov't  Virus Cultivation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).