Document 1004
 DOCN  M9541004
 TI    Relevance of the reactivity of HIV 1 positive sera with synthetic
       peptides for understanding antigenic differences between circulating
       strains.
 DT    9504
 AU    Cernescu C; Ruta SM; Stefan S. Nicolau, Institute of Virology,
       Bucharest, Romania.
 SO    Rev Roum Virol. 1993 Jul-Dec;44(3-4):175-86. Unique Identifier :
       AIDSLINE MED/95127474
 AB    Several problems have arisen concerning the detection methodology of
       sources and chains of HIV transmission based on molecular and antigenic
       relatedness of HIV isolates. Great progress in mapping virus genome or
       provirus, especially in identifying the changes in the env gene provides
       interesting data for molecular epidemiology. We address the same problem
       by the investigation of the reactivity of sera from HIV 1 seropositive
       children with two panels of synthetic peptides mimicking immunoreactive
       fragment of HIV1 glycoproteins in solid phase EIA. The samples proceed
       from two distinct settings: an orphanage in which nosocomial
       transmission was highly suspected and from different hospitals caring
       for HIV infected children. The synthetic peptides have sequences which
       reproduce two target regions: one in the principal neutralization domain
       of gp 120 (V3 loop) and the other at the COOH end of gp 41. Our results
       indicate that minimal differences in aminoacids flanking central
       conserved GPGRA region in V3 loop of gp 120 evidence strain specific
       antibodies while the COOH end of gp 41 reveals antibodies which link HIV
       1 strains in a broad fashion. Sera from HIV infected children collected
       in different settings recognize the same synthetic peptides panel with
       distinct frequencies suggesting circulation of different antigenic
       strains.
 DE    Amino Acid Sequence  Antigenic Variation/*IMMUNOLOGY  Binding,
       Competitive  Chi-Square Distribution  Child  Comparative Study
       Enzyme-Linked Immunosorbent Assay/METHODS  Human  HIV Antibodies/*BLOOD
       HIV Antigens/*IMMUNOLOGY  HIV Seropositivity/*IMMUNOLOGY  HIV
       Seroprevalence  HIV-1/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY  Molecular Sequence
       Data  Peptides/*IMMUNOLOGY  Romania/EPIDEMIOLOGY  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

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