Document 1012
 DOCN  M9541012
 TI    Genetic and antigenic variability of HIV type 1 in Brazil.
 DT    9504
 AU    Couto-Fernandez JC; Janssens W; Heyndrickx L; Motte J; Fransen K;
       Peeters M; Delaporte E; Galvao-Castro B; Piot P; van der Groen G;
       Advanced Public Health Laboratory, Centro de Pesquisas Goncalo,;
       Fundacao Oswaldo Cruz, Bahia CEP, Brazil.
 SO    AIDS Res Hum Retroviruses. 1994 Sep;10(9):1157-63. Unique Identifier :
       AIDSLINE MED/95127298
 AB    Six Brazilian strains of human immunodeficiency virus type 1 (HIV-1)
       were isolated from infected individuals residing in different regions of
       Brazil between 1987 and 1989. Phylogenetic analysis based on an 860-base
       pair env fragment, including V3, V4, V5, and the beginning of gp41,
       classified the Brazilian strains significantly in genotype B, with
       interhost distances between 5.9 and 13.1% (mean value, 10%). Amino acid
       sequence analysis of the V3 loop revealed that three strains contained
       the North American/European GPGR motif as the tip of the loop whereas in
       the other three strains proline (P) was substituted by tryptophan (W),
       methionine (M), or phenylalanine (F). A consensus peptide, Bra-cons, was
       designed containing GWGR as the tip of the loop. Serological reactivity
       to the Bra-cons peptide and other V3 peptides (MN, SF2, HBX2, RF, MAL,
       ELI, Z6, and a Cote d'Ivoire peptide, CI-cons) was compared for 114
       HIV-1-positive sera from Rio de Janeiro. Sixty-nine sera (60.5%) reacted
       with peptides belonging to genotype B, of which 10 sera also reacted
       with peptides belonging to genotype A (n = 7) and D (n = 3). Eighteen
       sera (15.8%) had binding antibodies to the Bra-cons peptide. A high
       number of sera (n = 43; 37.7%) had no antibodies to any of the V3
       peptides tested. This result suggests that HIV-1 variants with aberrant
       V3 loops may circulate in Rio de Janeiro.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*VIROLOGY  Amino Acid Sequence
       Brazil  Comparative Study  Enzyme-Linked Immunosorbent Assay  Gene
       Products, env/CHEMISTRY/*GENETICS  *Genes, env  Genotype  Geography
       Human  HIV Antibodies/IMMUNOLOGY  HIV-1/*GENETICS/IMMUNOLOGY/ISOLATION &
       PURIF  Molecular Sequence Data  *Phylogeny  Support, Non-U.S. Gov't
       Variation (Genetics)  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).