Document 1021
 DOCN  M9541021
 TI    Binding of glycoprotein 120 and peptides from the HIV-1 envelope by
       autoantibodies in mice with experimentally induced systemic lupus
       erythematosus and in patients with the disease.
 DT    9504
 AU    Bermas BL; Petri M; Berzofsky JA; Waisman A; Shearer GM; Mozes E;
       Experimental Immunology Branch, National Cancer Institute, NIH,;
       Bethesda, Maryland 20892.
 SO    AIDS Res Hum Retroviruses. 1994 Sep;10(9):1071-7. Unique Identifier :
       AIDSLINE MED/95127289
 AB    Systemic lupus erythematosus (SLE) and infection with the human
       immunodeficiency virus type 1 (HIV) are diseases that are characterized
       by immune dysregulation and autoantibody production. In this article we
       identify and characterize IgG antibodies from mice with SLE and SLE
       patients that bind HIV gp120 and HIV envelope-derived peptides. SLE can
       be induced in susceptible mouse strains by immunization with a human
       monoclonal anti-DNA antibody that bears a common idiotype designated
       16/6 Id. We tested sera from various strains of mice in which
       experimental SLE was induced by this method, as well as from 93 patients
       with SLE and 31 controls (17 healthy controls, 14 patients with other
       autoimmune diseases) for the presence of antibodies reactive to gp120 by
       an ELISA. Antibodies reactive with gp120 were produced by BALB/c,
       C3H.SW, AKR, and DBA/2 mice, all of which were 16/6 Id immunized and had
       experimental SLE. C57BL/6 mice, which are resistant to induction of SLE
       by this method, did not produce antibodies reactive with gp120 despite
       16/6 immunization. Forty-three percent of SLE patients made antibodies
       that bound to gp120 at titers greater than 1:40, whereas 12% of healthy
       control sera (p < or = 0.02) and 14% of patients with other autoimmune
       diseases contained such antibodies (p < or = 0.05). We delineated the
       specificity of this antibody activity by testing for reactivity to six
       HIV envelope peptides. In both mice and SLE patients, sera reactive with
       gp120 recognized the same three envelope peptides. Removal of the
       anti-DNA antibodies from the sera by DNA-agarose affinity purification
       did not change anti-gp120 specificity.
 DE    Amino Acid Sequence  Animal  Autoantibodies/*BLOOD/ISOLATION & PURIF
       Autoimmune Diseases/BLOOD/IMMUNOLOGY  Chromatography, Affinity
       Comparative Study  Enzyme-Linked Immunosorbent Assay  Human  HIV
       Envelope Protein gp120/*IMMUNOLOGY/METABOLISM  HIV-1/*IMMUNOLOGY  Lupus
       Erythematosus, Systemic/BLOOD/*IMMUNOLOGY  Mice  Molecular Sequence Data
       Peptides/CHEMISTRY/CHEMICAL SYNTHESIS/*IMMUNOLOGY  Recombinant
       Proteins/IMMUNOLOGY/METABOLISM  Reference Values  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       Viral Envelope Proteins/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).