Document 0021
 DOCN  M9550021
 TI    Production of Vibrio cholerae ghosts (VCG) by expression of a cloned
       phage lysis gene: potential for vaccine development.
 DT    9505
 AU    Eko FO; Szostak MP; Wanner G; Lubitz W; Institute of Microbiology and
       Genetics, University of Vienna,; Austria.
 SO    Vaccine. 1994 Oct;12(13):1231-7. Unique Identifier : AIDSLINE
       MED/95141687
 AB    The protein E-specific lysis mechanism of the Escherichia coli-specific
       bacteriophage PhiX174 was employed to produce Vibrio cholerae ghosts
       (VCG). VCG consist of both rounded and collapsed cells that have lost
       their cytoplasmic contents through an E-specific hole in the cell
       envelope. These ghosts are proposed as non-living material for
       immunization against cholera. A specific membrane anchor sequence was
       used to insert the human immunodeficiency virus type 1 (HIV-1) reverse
       transcriptase (RT) fusion protein into the cell envelope of V. cholerae.
       The identity of the expression products was confirmed by Western blot
       analysis employing an RT-specific monoclonal antibody. HIV-1 RT was
       chosen as a model for the purpose of evaluating heterologous gene
       expression in V. cholerae and the carrier potential of VCG.
       Intraperitoneal immunization of mice was used to evaluate the
       immunogenic potential of VCG. Preliminary results showed significant
       seroconversions to intact whole-cell vibrio antigens in mice immunized
       with VCG or a heat-killed whole-cell vibrio preparation.
 DE    Animal  Antibodies, Bacterial/BIOSYNTHESIS  Antibodies, Monoclonal
       Bacteriophage phi X 174/GENETICS  Cell Membrane/IMMUNOLOGY
       Cholera/PREVENTION & CONTROL  Cholera Vaccine/*ISOLATION & PURIF
       Cloning, Molecular  Genes, Viral  Human  HIV-1/ENZYMOLOGY/GENETICS
       Immunization  Mice  Microscopy, Electron  Plasmids  Recombinant Fusion
       Proteins/GENETICS  Reverse Transcriptase/GENETICS/IMMUNOLOGY  Support,
       Non-U.S. Gov't  Transformation, Genetic  Vaccines, Synthetic/ISOLATION &
       PURIF  Vibrio cholerae/*GENETICS/*IMMUNOLOGY/ULTRASTRUCTURE  Viral
       Proteins/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).