Document 0023
 DOCN  M9550023
 TI    Effect of a lipopeptidic formulation on macrophage activation and
       peptide presentation to T cells.
 DT    9505
 AU    Rouaix F; Gras-Masse H; Mazingue C; Diesis E; Ridel PR; Estaquier J;
       Capron A; Tartar A; Auriault C; Centre d'Immunologie des Maladies
       Transmissibles et Allergiques,; Unite mixte INSERM U167-CNRS 624, Lille,
       France.
 SO    Vaccine. 1994 Oct;12(13):1209-14. Unique Identifier : AIDSLINE
       MED/95141683
 AB    We studied a 45-69 lipopeptide obtained by N-terminal modification with
       a N epsilon-palmitoyl lysine residue of the 45-69 peptide derived from
       the nef protein of HIV. T cells from animals immunized intraperitoneally
       with 45-69 lipopeptide proliferated in vitro in the presence of 45-69
       peptide while no response was obtained after intraperitoneal
       immunization with 45-69 peptide. The efficiency of the 45-69 lipopeptide
       is supported by the covalent association to the N epsilon-palmitoyl
       lysine moiety. The immunogenicity of the 45-69 lipopeptide or of the
       unmodified peptide is dependent on the route of immunization but is not
       related to a mitogenic effect on cells or to an increase of the peptide
       antigenicity. Moreover, only 45-69 lipopeptide induces the secretion of
       cytokines such as IL-1, IL-6 and TNF-alpha by peritoneal macrophages.
       Finally, the use of 45-69 lipopeptide permits the activation of highly
       purified T cells without the addition of antigen-presenting cells. These
       results have implications for the formulation of synthetic vaccines.
 DE    Amino Acid Sequence  Animal  *Antigen Presentation
       Cytokines/BIOSYNTHESIS  Gene Products, nef/CHEMISTRY/GENETICS/IMMUNOLOGY
       HIV-1/GENETICS/IMMUNOLOGY  Immunization  In Vitro  Injections,
       Intraperitoneal  Injections, Subcutaneous
       Lipoproteins/CHEMISTRY/GENETICS/*IMMUNOLOGY  *Macrophage Activation
       Male  Molecular Sequence Data  Molecular Structure  Rats  Rats, Inbred
       Lew  Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY  Vaccines,
       Synthetic/ADMINISTRATION & DOSAGE/CHEMISTRY/ISOLATION &  PURIF  JOURNAL
       ARTICLE

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