Document 0029
 DOCN  M9550029
 TI    Phenotypic analysis of donor cells infiltrating the small intestinal
       epithelium and spleen during graft-versus-host disease.
 DT    9505
 AU    Schattenfroh NC; Hoffman RA; McCarthy SA; Simmons RL; Department of
       Surgery, University of Pittsburgh, Pennsylvania; 15261.
 SO    Transplantation. 1995 Jan 27;59(2):268-73. Unique Identifier : AIDSLINE
       MED/95141394
 AB    One of the principal target organs during graft-versus-host disease
       (GvHD) is the intestinal epithelium, although the reasons for the
       preferential involvement of particular organs in this disease are not
       known. This study analyzed the subset distribution of donor and host
       lymphocytes in the small intestinal epithelium and the spleen during
       GvHD in a parent (C57BL/6J) into F1 (C57BL/6JxDBA2/J F1) model. While
       the donor cell population in the spleen consisted of B and T cells, the
       donor cell population in the intestine contained only T cells during the
       course of GvHD. These infiltrating donor cells resembled the host
       intraepithelial lymphocytes (IELs), which are predominantly CD8+ T
       cells. This subset distribution of donor cells in the intestinal
       epithelium was remarkable since they originated from a donor splenocyte
       population containing few CD8+ lymphocytes. In addition, although the
       injected donor splenic T cells were virtually all alpha/beta TCR+,
       several months after GvHD induction more than 30% of the donor cells in
       the intestine were gamma/delta TCR+, thereby resembling the host IELs
       not only in their expression of CD4 and CD8, but also in their TCR
       expression. In contrast, no gamma/delta TCR+ donor cells were detectable
       in the spleen of GvHD mice. The subset distribution of donor and host
       IELs remained constant throughout the disease, while in the spleen a
       decrease of both donor and host B cells and a temporary increase of both
       donor and host CD8+ cells was observed. These findings demonstrate that
       in a given target organ during GvHD the disease process affects both
       donor and host lymphoid populations. In addition the different tissue
       microenvironments eventually lead to donor cell repopulation with a
       subset distribution similar to the host natural lymphoid population of
       the particular target organ.
 DE    Animal  B-Lymphocyte Subsets/IMMUNOLOGY  Comparative Study  CD4-CD8
       Ratio  CD8-Positive T-Lymphocytes/IMMUNOLOGY  Disease Models, Animal
       Epithelium/CYTOLOGY/IMMUNOLOGY  Graft vs Host
       Disease/*IMMUNOLOGY/*PATHOLOGY  Intestinal Mucosa/CYTOLOGY/IMMUNOLOGY
       Intestine, Small/*CYTOLOGY/*IMMUNOLOGY  Lymphocyte Subsets/*IMMUNOLOGY
       Male  Mice  Mice, Inbred C57BL  Mice, Inbred DBA  Phenotype  Receptors,
       Antigen, T-Cell, gamma-delta/ANALYSIS/IMMUNOLOGY
       Spleen/*CYTOLOGY/*IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  T-Lymphocyte Subsets/IMMUNOLOGY  Tissue Donors  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).