Document 0047
 DOCN  M9550047
 TI    Comparative study of single and nested PCR for the detection of proviral
       HIV2 DNA.
 DT    9505
 AU    Loussert-Ajaka I; Simon F; Farfara I; Houhou N; Couto-Fernandez J; Dazza
       MC; Brun-Vezinet F; Laboratoire de Virologie, Hopital Bichat-Claude
       Bernard, Paris.
 SO    Res Virol. 1994 Sep-Oct;145(5):337-42. Unique Identifier : AIDSLINE
       MED/95140936
 AB    We have tested seven pairs of primers for the detection of HIV2 DNA by
       single PCR in positive cultures from 21 infected patients. Four of these
       primer pairs were then used in a comparative study of single and nested
       PCR for the detection of HIV2 in fresh lymphocytes from 33 patients
       infected by the virus. HIV2 DNA was detected in 17 of the 33 patients
       (51.5%) by single PCR and 29/33 (88%) by nested PCR. All the patients
       negative in both nested and single PCR were asymptomatic and had CD4+
       lymphocyte counts of at least 500 x 10(6)/l. This lack of PCR
       sensitivity for the detection of proviral HIV2 DNA in fresh lymphocytes
       cannot be totally attributed to genetic variability and may be related
       to a low viral load in asymptomatic HIV2-infected patients.
 DE    Africa, Western/EPIDEMIOLOGY  CD4 Lymphocyte Count  DNA Primers  DNA,
       Viral/*BLOOD  HIV Seropositivity/EPIDEMIOLOGY/GENETICS/*VIROLOGY
       HIV-1/GENETICS  HIV-2/GENETICS/*ISOLATION & PURIF  Leukocytes,
       Mononuclear/*VIROLOGY  Polymerase Chain Reaction/*METHODS
       Proviruses/GENETICS/*ISOLATION & PURIF  Sensitivity and Specificity
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).