Document 0065
 DOCN  M9550065
 TI    Immunization with irradiated Plasmodium berghei sporozoites induces IL-2
       and IFN gamma but not IL-4.
 DT    9505
 AU    White KL; Jarboe DL; Krzych U; Department of Biology, Catholic
       University of America,; Washington, DC 20064.
 SO    Parasite Immunol. 1994 Sep;16(9):479-91. Unique Identifier : AIDSLINE
       MED/95140494
 AB    Protective immunity against Plasmodium induced by immunization with
       irradiated sporozoites (SPZ) depends on both humoral and cellular
       responses. Although circumsporozoite protein (CSP)-specific cytolytic T
       lymphocyte responses have been established as an effector system, other
       cell types are required for protection. We have previously demonstrated
       that although protective immunity and T cell proliferative reactivity to
       SPZ are mouse strain- and SPZ dose-dependent, no correlation between the
       two responses could be found. Since protective immunity involves
       functionally diverse T cell subsets, we asked whether the discordance
       between proliferative responses to SPZ and protective immunity might
       have resulted from selective activation of either the Th1 or Th2 cell
       subset. Protective immunity, in vitro proliferative responses, and
       lymphokine production were tested in BALB/c, C57Bl/6, and C3H/HeN mice
       immunized according to different SPZ regimens. The levels of IL-2
       paralleled the proliferative reactivities in each mouse strain examined.
       Although IFN gamma levels were present in the unprimed lymphocyte
       cultures, they increased following each SPZ immunization, in C57Bl/6,
       moderate in C3H/HeN, and lowest in BALB/c splenic cultures.
       Surprisingly, no IL-4 was detected in splenic cultures from any mouse
       strain during proliferative activity or protective immunity. In
       contrast, elevated IL-6 production was noted after each immunization,
       regardless of the protective status and it correlated with anti-CSP IgG
       serum levels. These data establish that lymphokine profiles
       corresponding primarily to the Th1 cells were induced by immunization
       with P. berghei SPZ and that IL-4 secreting T cells were not induced by
       the SPZ-stage berghei antigens.
 DE    Animal  Antibodies, Protozoan/ANALYSIS  Cells, Cultured  Female
       Immunization  Interferon Type II/*BIOSYNTHESIS
       Interleukin-2/*BIOSYNTHESIS  Interleukin-4/*BIOSYNTHESIS  Lymphocyte
       Transformation  Malaria/IMMUNOLOGY/*PREVENTION & CONTROL  Mice  Mice,
       Inbred BALB C  Mice, Inbred C3H  Mice, Inbred C57BL  Plasmodium
       berghei/*IMMUNOLOGY/RADIATION EFFECTS  RNA, Messenger/ANALYSIS  Th1
       Cells/*IMMUNOLOGY  Th2 Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).