Document 0107
 DOCN  M9550107
 TI    Overexpression of IL-10 in atopic dermatitis. Contrasting cytokine
       patterns with delayed-type hypersensitivity reactions.
 DT    9505
 AU    Ohmen JD; Hanifin JM; Nickoloff BJ; Rea TH; Wyzykowski R; Kim J; Jullien
       D; McHugh T; Nassif AS; Chan SC; et al; Division of Dermatology,
       University of California, School of; Medicine, Los Angeles 90024.
 SO    J Immunol. 1995 Feb 15;154(4):1956-63. Unique Identifier : AIDSLINE
       MED/95138541
 AB    The skin lesions of patients with atopic dermatitis provide a model to
       study immunoregulation in human allergy. To determine the local cytokine
       pattern of cells present (both endogenous and recruited) at the site of
       disease, we extracted RNA from skin biopsy specimens from patients with
       atopic dermatitis, allergic contract dermatitis, and positive tuberculin
       reactions and used PCR to assay for cytokine mRNA. cDNAs were normalized
       to the intensity of the CD3 delta PCR product as a marker of T cell
       mRNA. We found overexpression of IL-10 mRNA in atopic dermatitis
       lesions, in comparison with allergic contact dermatitis lesions and
       tuberculin reactions. In contrast, IL-4 mRNA was most strongly expressed
       in allergic contact dermatitis lesions and IFN-gamma mRNA was the
       predominant cytokine in tuberculin reactions. Using an anti-IL-10 mAb
       with immunoperoxidase, we localized IL-10 protein to large mononuclear
       cells in the dermal infiltrate of atopic lesions. After immunomagnetic
       sorting of mononuclear cell populations from PBMC of atopic dermatitis
       subjects, IL-10 mRNA as measured by PCR was found to be strongly
       expressed in CD14+ cells. Spontaneous release of IL-10 from PBMC-derived
       adherent cells was greater in atopic dermatitis donors than normal
       controls. We therefore renormalized skin biopsy cDNA according to the
       level of beta-actin PCR product, as a marker of total cellular mRNA, and
       found by PCR that IL-10 was nevertheless greatest in atopic dermatitis
       subjects. We conclude that the relative overexpression of IL-10 in
       atopic dermatitis greatest in atopic dermatitis subjects. We conclude
       that the relative overexpression of IL-10 in atopic dermatitis may
       contribute to the up-regulation of humoral responses and the
       down-regulation of Th1 responses.
 DE    Adult  Aged  Aged, 80 and over  Biopsy  Dermatitis,
       Atopic/GENETICS/*METABOLISM  Dermatitis, Contact/GENETICS/METABOLISM
       DNA, Complementary/GENETICS  Female  Gene Expression  Human
       Hypersensitivity, Delayed/*IMMUNOLOGY  Interferon Type
       II/BIOSYNTHESIS/GENETICS  Interleukin-10/*BIOSYNTHESIS/GENETICS
       Interleukins/BIOSYNTHESIS/GENETICS  Male  Middle Age
       Monocytes/IMMUNOLOGY/METABOLISM  Polymerase Chain Reaction  RNA,
       Messenger/ANALYSIS  Skin/PATHOLOGY  Skin Tests  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Th2 Cells/IMMUNOLOGY/METABOLISM
       Tuberculosis, Pulmonary/GENETICS/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).