Document 0110
 DOCN  M9550110
 TI    Superantigen-induced human CD4+ helper/killer T cell phenomenon.
       Selective induction of Th1 helper/killer T cells and application to
       tumor immunotherapy.
 DT    9505
 AU    Kuge S; Miura Y; Nakamura Y; Mitomi T; Habu S; Nishimura T; Department
       of Surgery, Tokai University School of Medicine,; Isehara, Japan.
 SO    J Immunol. 1995 Feb 15;154(4):1777-85. Unique Identifier : AIDSLINE
       MED/95138521
 AB    Human CD4+ T cells activated with staphylococcal enterotoxin A (SEA)
       were fractionated by Percoll discontinuous density gradient
       centrifugation to enrich SEA-reactive CD4+ T cells. The SEA-reactive
       CD4+ T cells showed significant cytotoxicity, so-called
       superantigen-dependent cell-mediated cytotoxicity, against SEA-coated
       class II-positive tumor cells. During lysis of SEA-coated tumor cells,
       SEA-reactive CD4+ T cells produced high levels of IL-2 and IFN-gamma but
       not IL-4 in an Ag-specific manner. The skewing of human CD4+ T cells to
       Th1-type helper/killer T cells was also demonstrated when SEA-reactive
       CD4+V beta 5.3+ clonal T cells were cultured with SEA, but not with PHA
       or OKT3 mAb. Interestingly, the generation of SEA-reactive helper/killer
       T cells was negatively regulated by IL-4, but up-regulated by IL-12. The
       SEA-reactive CD4+ helper/killer T cells were able to generate from PBMC
       of tumor patients and could be expanded to 10(9) levels in a 7-day
       culture. The SEA-reactive CD4+ helper/killer T cells were specifically
       targeted to c-erbB-2 positive human colon cancer cells using
       SEA-conjugated-anti-c-erbB-2 mAb. These results initially demonstrated
       that SEA-activated human CD4+ T cells are a Th1 type of Th cell that has
       both helper and killer functions which may be useful for adoptive tumor
       immunotherapy in combination with SEA-conjugated antitumor mAb.
 DE    Antibodies, Monoclonal/IMMUNOLOGY  Cells, Cultured  Cytotoxicity,
       Immunologic  Enterotoxins/IMMUNOLOGY  Histocompatibility Antigens Class
       II  Human  Interferon Type II/BIOSYNTHESIS  Interleukin-2/BIOSYNTHESIS
       Killer Cells/*IMMUNOLOGY  *Lymphocyte Transformation  Proto-Oncogene
       Proteins c-erbB-2/IMMUNOLOGY  Superantigens/*IMMUNOLOGY  Support,
       Non-U.S. Gov't  Th1 Cells/*IMMUNOLOGY  Tumor Cells, Cultured  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).