Document 0121
 DOCN  M9550121
 TI    Promiscuous and specific binding of HIV peptides to HLA-DR1 and DR103.
       Impact on T-cell repertoire of nonimmunized individuals.
 DT    9505
 AU    Praud C; Jurcevic S; L'Faqihi FE; Guiraud M; de Preval C; Thomsen M;
       INSERM U395, Toulouse, France.
 SO    Hum Immunol. 1994 Sep;41(1):56-60. Unique Identifier : AIDSLINE
       MED/95137782
 AB    The binding of immunogenic peptides to DR molecules is influenced by
       residues that point into the peptide-binding groove. The T-cell response
       toward a peptide complexed to an MHC molecule depends on the presence of
       a sufficient number of T cells reactive with peptide-MHC complex on the
       surface of APCs. From 96 overlapping HIV peptides, we have selected 11
       that show a significant binding to either DR1, DR103, or both. These two
       DR molecules are identical except for three amino acids at positions 67,
       70, and 71 on the beta chain. Peptide-specific T-cell lines and clones
       were generated with cells from nonimmunized donors homozygous for DR1 or
       DR103 by using either individual peptides or peptide pools for the in
       vitro priming. Three of the peptides induced T-cell-specific
       proliferative response in both individuals, and these peptides were not
       among those with highest affinity. Most of the peptides induced strong
       responses against autologous APCs. This might reflect cross-reactivity
       between HIV and self-peptides. Definition of peptides that both show
       promiscuous binding to DR and elicit a strong T-cell response is
       important for design of efficient synthetic vaccines.
 DE    AIDS Vaccines/IMMUNOLOGY  Cell Line  Human  HIV Antigens/*IMMUNOLOGY
       HIV Envelope Protein gp120/METABOLISM  HLA-DR Antigens/*METABOLISM
       HLA-DR1 Antigen/METABOLISM  Lymphocyte Transformation/IMMUNOLOGY
       Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).