Document 0124
 DOCN  M9550124
 TI    Targeting of pancreatic islets of severe combined immunodeficient mice
       by passive transfer of allogeneic spleen cells from non-obese diabetic
       mice.
 DT    9505
 AU    Reddy S; Liu W; Elliott RB; Department of Paediatrics, University of
       Auckland School of; Medicine, New Zealand.
 SO    Immunol Cell Biol. 1994 Oct;72(5):390-7. Unique Identifier : AIDSLINE
       MED/95137690
 AB    The precise role of immune cells in beta cell killing and their manner
       of invasion of pancreatic islets in insulin-dependent diabetes mellitus
       (IDDM) are unclear. We have attempted to target pancreatic islets of
       severe-combined immunodeficient (SCID) mice with spleen cells from
       diabetic and non-diabetic female non-obese diabetic (NOD) mice given
       i.p. or i.v. Pancreatic, liver and kidney sections of SCID mice were
       assessed histologically for the presence of donor cells. The presence of
       raised levels of serum Ig was also used as an index of engraftment of
       donor cells in the periphery of SCID mice. All six SCID mice which
       received i.v. spleen cells from normal Swiss mice died within 2 weeks
       from graft versus host disease (GVHD) whereas five out of nine mice
       survived for 30 days after i.p. injection. No deaths were recorded after
       i.v. or i.p. injection of spleen cells from NOD mice. Pancreatic islets
       of four out of six SCID recipients of diabetic and three out of five
       recipients of non-diabetic spleen cells following i.p. injection showed
       lymphocytic infiltrates in the peri-islet and perivascular regions. All
       SCID mice which received i.v. spleen cells from diabetic (six SCID
       recipients) and non-diabetic NOD mice (seven SCID recipients) showed
       peri-islet and perivascular infiltrates in their pancreas.
       Immunohistochemical analysis showed that the islet engrafted cells were
       of CD4 and CD8 phenotype. Donor cells were also observed in the exocrine
       pancreas of some recipients. A majority of mice showed various degrees
       of lymphocytic aggregates in the perivascular regions of the liver but
       not in the kidney.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Animal  CD4-Positive T-Lymphocytes/PATHOLOGY  CD8-Positive
       T-Lymphocytes/PATHOLOGY  Diabetes Mellitus,
       Insulin-Dependent/*IMMUNOLOGY  Female  Graft vs Host
       Disease/IMMUNOLOGY/PATHOLOGY  *Immunization, Passive  Islets of
       Langerhans/*IMMUNOLOGY  Kidney/PATHOLOGY  Liver/PATHOLOGY  Male  Mice
       Mice, Inbred NOD  Mice, SCID  Pancreas/PATHOLOGY  Severe Combined
       Immunodeficiency/*IMMUNOLOGY/PATHOLOGY  Spleen/CYTOLOGY/*IMMUNOLOGY
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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