Document 0125
 DOCN  M9550125
 TI    Effects of irradiation, glucocorticoid and FK506 on cell-surface antigen
       expression by rat thymocytes: a three-colour flow cytofluorometric
       analysis.
 DT    9505
 AU    Tsuchida M; Konishi M; Takai K; Naito K; Fujikura Y; Fukumoto T;
       Department of Urology, Yamaguchi University School of Medicine,; Japan.
 SO    Immunology. 1994 Nov;83(3):469-75. Unique Identifier : AIDSLINE
       MED/95137679
 AB    The expression of T-cell antigen receptor (TCR) alpha beta was
       investigated in rat CD4- CD8- thymocytes during thymic reconstitution
       after the exposure of animals to irradiation or glucocorticoid. The
       effect of the immunosuppressant FK506 on the expression of TCR alpha
       beta in rat CD4- CD8- thymocytes was also examined. The percentage of
       CD4- CD8- thymocytes constituted 2.6% of total thymocytes and that of
       CD4- CD8- TCR alpha beta high cells constituted 12.6% of CD4- CD8-
       thymocytes in normal adult Lewis rats. The percentage of CD4- CD8- TCR
       alpha beta high cells increased during thymic reconstitution after
       irradiation, and maximally constituted 28.6% of CD4- CD8- thymocytes on
       day 7. Similar results were obtained during thymic reconstitution after
       glucocorticoid treatment. In contrast, continuous treatment with FK506
       for 7 days markedly decreased not only the percentages of CD4+ CD8- TCR
       alpha beta high and CD4- CD8+ TCR alpha beta high thymocytes, but also
       that of CD4- CD8- TCR alpha beta high thymocytes. These results indicate
       that rat CD4- CD8- thymocytes contain a subpopulation of mature (TCR
       alpha beta high) cells. The possible implications of the existence of
       this subpopulation with regard to thymocyte differentiation and
       maturation are discussed.
 DE    Animal  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Dexamethasone/PHARMACOLOGY  Flow Cytometry
       FK-506/*PHARMACOLOGY  Male  Rats  Rats, Inbred Lew  Receptors, Antigen,
       T-Cell, alpha-beta/*IMMUNOLOGY  Thymus Gland/CYTOLOGY/DRUG
       EFFECTS/*IMMUNOLOGY/RADIATION EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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