Document 0126
 DOCN  M9550126
 TI    Role of IL-2 and IL-4 in exacerbations of murine antigen-induced
       arthritis.
 DT    9505
 AU    Jacobs MJ; van den Hoek AE; van Lent PL; van de Loo FA; van de Putte LB;
       van den Berg WB; Department of Rheumatology, University Hospital
       Nijmegen, The; Netherlands.
 SO    Immunology. 1994 Nov;83(3):390-6. Unique Identifier : AIDSLINE
       MED/95137667
 AB    In this study the roles of different T-cell subsets, and produced
       cytokines, were investigated in an animal model for acute exacerbations.
       Flare-up reactions are inducible in the chronic phase of a smouldering
       antigen-induced inflammation by injection of a small amount of an
       antigen into a hyper-reactive knee joint. In vivo treatment with
       anti-CD4 monoclonal antibodies (mAb) almost totally blocked the flare
       reaction, whereas anti-CD8 treatment did not exert any effect. The role
       of T-helper 1 (Th1) cells in delayed-type hypersensitivity-resembling
       diseases is generally entitled proinflammatory, whereas Th2 cells act in
       an anti-inflammatory manner. To investigate the role of these T-cell
       subsets in flare-up reactions, anti-interleukin-2 (IL-2) and anti-IL-4
       mAb treatments were performed. Anti-IL-2 treatment partly blocked the
       flare reaction, and anti-IL-4 treatment, although the result was
       unexpected, blocked the flare more efficiently. Furthermore, when human
       recombinant IL-2 (hrIL-2) and murine recombinant IL-4 (mrIL-4) were
       co-injected with the antigen to test their ability respectively to
       potentiate or down-regulate the flare reaction, both cytokines
       demonstrated additional pro-inflammatory effects, although hrIL-2 was
       more potent than mrIL-4. The mere effect of hrIL-2 and mrIL-4 was
       studied by direct injection into a hyperreactive joint. No flare-up
       reaction or cell-influx could be induced, suggesting that other
       mediators are needed to exert pro-inflammatory effects of IL-2 or IL-4.
       We conclude that not only Th1 cells, but also Th2 lymphocytes (at least
       regarding IL-4 production) may play a pro-inflammatory role in flare-up
       reactions of chronic arthritis. Considering therapeutic application of
       Th2 cell-derived cytokines, one should be aware of the possible
       pro-inflammatory potential of IL-4.
 DE    Acute Disease  Animal  Antibodies, Monoclonal/ADMINISTRATION & DOSAGE
       Arthritis/*IMMUNOLOGY  Female  Immunization  Interleukin-2/PHYSIOLOGY
       Interleukin-4/PHYSIOLOGY  Interleukins/*PHYSIOLOGY  Mice  Mice, Inbred
       C57BL  Support, Non-U.S. Gov't  T-Lymphocytes,
       Helper-Inducer/*IMMUNOLOGY  Th1 Cells/IMMUNOLOGY  Th2 Cells/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).