Document 0128
 DOCN  M9550128
 TI    A Th1 cell line (3E9.1) from resistant A/J mice inhibits induction of
       macrophage procoagulant activity in vitro and protects against MHV-3
       mortality in vivo.
 DT    9505
 AU    Chung S; Gorczynski R; Cruz B; Fingerote R; Skamene E; Perlman S;
       Leibowitz J; Fung L; Flowers M; Levy G; University of Toronto, Canada.
 SO    Immunology. 1994 Nov;83(3):353-61. Unique Identifier : AIDSLINE
       MED/95137662
 AB    Induction of immune coagulants has been implicated in the pathogenesis
       of murine hepatitis virus strain 3 (MHV-3)-induced fulminant hepatic
       necrosis. Previous work from our laboratory has shown that the induction
       of procoagulant activity (PCA) correlates with the
       resistance/susceptibility to disease in inbred and recombinant inbred
       (RI) strains of mice. Macrophages from susceptible, but not resistant,
       strains of mice expressed increased levels of PCA in response to MHV-3
       stimulation. T lymphocytes, however, had a marked regulatory role in the
       final expression of macrophage PCA. CD3+ CD4+ CD8- lymphocytes from RI
       H-2 compatible susceptible mice were able to instruct macrophages from
       susceptible mice to express significantly augmented levels of PCA,
       whereas CD3+ lymphocytes from RI H-2 compatible MHV-3-immunized
       resistant mice were able to suppress induction of PCA. In this present
       study, T-cell lines were derived from draining popliteal lymph nodes
       from resistant A/J mice, which had been immunized with MHV-3. All T-cell
       lines showed marked proliferation to MHV-3 and MHV-JHM which was major
       histocompatibility complex (MHC) restricted. All cell lines were CD3+,
       four of these were CD4+ and one was CD8+. All of the CD4+ cell lines
       produced IL-2 and two produced interferon-gamma (IFN-gamma), consistent
       with the Th1 cytokine profile. One cell line (3E9.1) was able to inhibit
       the induction of macrophage PCA through production of a soluble factor
       although cell-to-cell contact could not be excluded. This CD4+ T-cell
       line conferred protection to infected and susceptible AXB8 mice. These
       results demonstrate that the existence of a Th1 subpopulation of cells
       with a regulatory effect on macrophage PCA induction in MHV-3-infected
       mice contributes to the resistance of the A/J strain of mice to MHV-3
       infection.
 DE    Animal  Base Sequence  Blood Coagulation  Cell Line  Coronavirus
       Infections/*IMMUNOLOGY  Disease Susceptibility  DNA Primers
       Gastroenteritis Virus, Murine/*IMMUNOLOGY  Hepatitis, Viral,
       Animal/*IMMUNOLOGY  Immunity, Natural  Macrophage Activation/*IMMUNOLOGY
       Macrophages/PHYSIOLOGY  Mice  Mice, Inbred C57BL  Mice, Inbred Strains
       Molecular Sequence Data  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  Th1 Cells/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).