Document 0129
 DOCN  M9550129
 TI    Protection against tuberculosis by passive transfer with T-cell clones
       recognizing mycobacterial heat-shock protein 65.
 DT    9505
 AU    Silva CL; Silva MF; Pietro RC; Lowrie DB; Department of Parasitology,
       Microbiology and Immunology, School; of Medicine of Ribeirao Preto,
       University of Sao Paulo, Brazil.
 SO    Immunology. 1994 Nov;83(3):341-6. Unique Identifier : AIDSLINE
       MED/95137660
 AB    We have previously shown that mice vaccinated by injection with J774
       macrophage-like tumour cells that expressed Mycobacterium leprae
       heat-shock protein (hsp) 65 as a transgene had acquired a remarkably
       high degree of protection against subsequent challenge with virulent M.
       tuberculosis. We show here that antigen-specific T cells cloned from
       spleens of such vaccinated animals can transfer a high level of
       protection to non-vaccinated recipients. The most efficient cells were
       of T-cell receptor (TCR) alpha beta+ and CD4- CD8+ type and specifically
       lysed mycobacteria-infected macrophages. These findings are consistent
       with the importance for protective immunity of engaging the endogenous
       antigen-presenting pathway to bias the immune response towards a
       cytolytic action against a mycobacterial antigen that is expressed at
       the surface of infected macrophages. TCR gamma delta+ and TCR alpha
       beta+ cells interacted synergistically.
 DE    Animal  Antigen-Presenting Cells/IMMUNOLOGY  Antigens,
       Bacterial/ADMINISTRATION & DOSAGE  Chaperonins/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Heat-Shock Proteins/*IMMUNOLOGY
       *Immunization, Passive  Mice  Mice, Inbred BALB C  Mycobacterium
       leprae/*IMMUNOLOGY  Receptors, Antigen, T-Cell, alpha-beta/IMMUNOLOGY
       Receptors, Antigen, T-Cell, gamma-delta/IMMUNOLOGY  Support, Non-U.S.
       Gov't  T-Lymphocytes/*IMMUNOLOGY  Tuberculosis/*PREVENTION & CONTROL
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).