Document 0131
 DOCN  M9550131
 TI    Antibodies to human and non-human primate cellular and culture medium
       components in macaques vaccinated with the simian immunodeficiency
       virus.
 DT    9505
 AU    Bergmeier LA; Walker J; Tao L; Cranage M; Lehner T; Division of
       Immunology, United Medical and Dental School, Guy's; Hospital, London,
       UK.
 SO    Immunology. 1994 Oct;83(2):213-20. Unique Identifier : AIDSLINE
       MED/95137639
 AB    Inactivated simian immunodeficiency virus (SIV) grown in a human T-cell
       line induces protection from infection by the virus in macaques.
       However, observations that immunization with uninfected human T cells or
       with SIV-1 prepared in human T cells can also induce protection, has
       raised the possibility that protective antigens could be of human
       cellular origin. Sera from animals immunized with fixed infected and
       uninfected human T cells, as well as from animals immunized with
       partially purified cell-free SIV have been examined for their ability to
       bind to human and macaque peripheral blood mononuclear cells (PBMC) and
       to-components present in fetal calf serum (FCS) in which the cells were
       grown. Analysis by flow cytometry suggests that antibodies to human cell
       surface antigens can be elicited with both inactivated SIV grown in
       human T cells and by uninfected T cells. There was a significant
       association between the presence of anti-cell antibodies and protection
       from infection. However, anti-cell surface antibodies were not detected
       with macaque mononuclear cells by flow cytometry or by
       immunoprecipitation, unless these cells were first treated with FCS or
       activated by a mitogen. Immunoprecipitation of resting human PBMC with
       sera from immunized animals suggests the presence of antibodies to class
       I heavy and light chains [beta 2-microglobulin (beta 2 m)] and to bovine
       beta 2m, which may originate in FCS used to grow the cell line.
       Antibodies to CD4 were also found in sera from animals immunized with
       SIV grown in human T cells. We suggest that human cellular components
       augmented by FCS elicit anti-class I heavy chain, beta 2m, CD4 and FCS
       antibodies which may be responsible for protection against SIV infection
       in macaques.
 DE    beta 2-Microglobulin/IMMUNOLOGY  Animal  Antibodies, Viral/*BIOSYNTHESIS
       Antigens, CD4/IMMUNOLOGY  Antigens, Surface/IMMUNOLOGY  Antigens,
       Viral/*IMMUNOLOGY  Blood Proteins/IMMUNOLOGY  Culture Media  Flow
       Cytometry  Macaca  Precipitin Tests  SIV/*IMMUNOLOGY
       T-Lymphocytes/*IMMUNOLOGY  *Vaccination  JOURNAL ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).