Document 0134
 DOCN  M9550134
 TI    Locomotor phenotypes of unstimulated CD45RAhigh and CD45ROhigh CD4+ and
       CD8+ lymphocytes in three-dimensional collagen lattices.
 DT    9505
 AU    Friedl P; Noble PB; Shields ED; Zanker KS; Faculty of Dentistry, McGill
       University, Montreal, Quebec,; Canada.
 SO    Immunology. 1994 Aug;82(4):617-24. Unique Identifier : AIDSLINE
       MED/95137625
 AB    The spontaneous locomotion of immunomagnetically isolated resting CD4+
       and CD8+ lymphocytes in three-dimensional collagen gels was recorded by
       time-lapse videomicroscopy. Two-dimensional projections of the paths of
       randomly selected individual cells were digitized, plotted and
       quantitatively analysed. Among five different donors 46 +/- 10% of CD4+
       and CD8+ lymphocytes (n = 180) showed initial spontaneous locomotion
       (individual speed 5-25 microns/min; mean speed CD4+ 5.2 +/- 3.6 microns,
       CD8+ 3.23 +/- 2.72 microns). Active CD4+ cells were constantly migrating
       for more than 4 hr, whereas CD8+ lymphocytes significantly slowed down
       after 60-90 min in the collagen gel (P < 0.003). Quantitative analysis
       of the paths indicated at least three migratory phenotypes: (1)
       spontaneously locomoting cells exhibiting high speed and low frequency
       of stopping; (2) a major non-motile fraction without significant
       displacement; and (3) a subpopulation within CD4+ and CD8+ cells with
       intermediate activity of speed and stopping. Further subtype analysis of
       immunomagnetically isolated CD45RAhigh/ROlow or CD45RAlow/ROhigh
       lymphocytes showed that more than 90% of CD4+ CD45RAhigh/ROlow cells
       were actively locomoting. In contrast, only 20% of the CD4+
       CD45ROhigh/RAlow phenotype showed spontaneous motility to a limited
       degree. The data indicate that resting CD4+ and CD8+ lymphocytes
       comprise further locomotory subpopulation related to the expression of
       different CD45 isoforms.
 DE    Antigens, CD45/*ANALYSIS  Cell Movement/IMMUNOLOGY  Cells, Cultured
       *Collagen  CD4-Positive T-Lymphocytes/*PHYSIOLOGY  CD8-Positive
       T-Lymphocytes/*PHYSIOLOGY  Extracellular Matrix/IMMUNOLOGY  Human
       Kinetics  Microscopy, Video  Support, Non-U.S. Gov't  T-Lymphocyte
       Subsets/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).