Document 0135
 DOCN  M9550135
 TI    Phenotypic and functional heterogeneity of murine intestinal
       intraepithelial lymphocytes defined by cell density: implications for
       route of differentiation and responsiveness to proliferation induction.
 DT    9505
 AU    Hamad M; Klein JR; Department of Biological Science, University of
       Tulsa, Oklahoma; 74104.
 SO    Immunology. 1994 Aug;82(4):611-6. Unique Identifier : AIDSLINE
       MED/95137624
 AB    The phenotype and function of murine intestinal intraepithelial
       lymphocytes (IEL) was studied in a Percoll-fractionated preparation that
       consisted of low-density cells which migrated to the 40-50% Percoll
       interface (IEL-40), medium-density cells which migrated to the 50-55%
       interface (IEL-50), and high-density cells which migrated to the 55-70%
       interface (IEL-55). IEL-40 and IEL-50 cells, the subsets phenotypically
       most similar to mature IEL, consisted of CD3+ T cells that included CD4-
       CD8+ and CD4+ CD8+ cells; CD4+ CD8- cells were present only in the
       IEL-50 fraction. T-cell receptor (TcR)alpha beta and TcR gamma delta
       cells were present in both IEL-40 and IEL-50 fractions. In contrast,
       most IEL-55 were CD3-, heat-stable antigen (HSA)+ cells that were not B
       cells; some IEL-55 cells were CD3lo HSA- or CD3lo HSA+ suggesting that
       IEL-55 are immature T cells. TcR alpha beta but not TcR gamma delta was
       expressed in the IEL-55 fraction. All three IEL fractions consisted of
       both CD8 alpha alpha and CD8 alpha beta cells. There was considerable
       functional heterogeneity between the three IEL fractions such that
       CD3-induced proliferation was greatest for IEL-50 cells and least for
       IEL-55 cells; that activity correlated with the proportion of Thy-1+
       cells within the fractions. Both IEL-40 and IEL-50 fractions contained
       activated cytotoxic T lymphocytes (CTL) that were 8-16-fold more lytic
       than IEL-55 cells. That IEL-55 cells may be precursors of some IEL-40
       and IEL-50 cells was demonstrated by a shift in cell density and by an
       increase in proportions of cells expressing markers of IEL-40 and IEL-50
       cells following in vitro stimulation via CD3. The relevance of these
       findings to differences in functional activities reported for murine IEL
       is discussed.
 DE    Animal  Antigens, CD3/IMMUNOLOGY  Antigens, Surface/ANALYSIS  Cell
       Division/IMMUNOLOGY  Centrifugation, Density Gradient  Cytotoxicity,
       Immunologic  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Epithelium/IMMUNOLOGY  Intestinal
       Mucosa/*IMMUNOLOGY  Lymphocyte Transformation/*IMMUNOLOGY  Mice  Mice,
       Inbred C57BL  Mice, Inbred DBA  Receptors, Antigen, T-Cell,
       alpha-beta/ANALYSIS  Receptors, Antigen, T-Cell, gamma-delta/ANALYSIS
       Spleen/IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocyte Subsets/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).