Document 0136
 DOCN  M9550136
 TI    Infiltration of CD4+ CD8+ T cells, and expression of ICAM-1, Ia
       antigens, IL-1 alpha and TNF-alpha in the skin lesion of BALB/c mice
       undergoing repeated infestations with nymphal Ixodes ricinus ticks.
 DT    9505
 AU    Mbow ML; Rutti B; Brossard M; Institute of Zoology, University of
       Neuchatel, Switzerland.
 SO    Immunology. 1994 Aug;82(4):596-602. Unique Identifier : AIDSLINE
       MED/95137622
 AB    The skin cellular immune response of BALB/c mice was examined during
       three successive infestations with nymphal Ixodes ricinus ticks. An
       immunohistochemical analysis of skin cryostat sections 72 hr post-tick
       attachment revealed that CD4+ T cells outnumbered CD8+ T cells in all
       infestations. The CD4+:CD8+ T-cell ratio was 2.2:1 in the primary
       infestation, then increased to 3.2:1 and 4.7:1 in the secondary and
       tertiary infestations. No B lymphocytes (CD45R) were detected in the
       skin of control and infested mice. A positive staining of intercellular
       adhesion molecule-1 (ICAM-1) on vascular endothelial cells, dendritic
       cells and some other mononuclear cells was observed in the dermis. Also,
       a strong positive staining of Ia antigens on dendritic cells and
       infiltrated mononuclear cells was noted. The staining pattern was more
       intense and positive cells increased in number in the skin of
       re-infested mice compared to the primary infestation. In addition, cells
       such as epidermal keratinocytes, dermal dendritic cells and infiltrated
       mononuclear cells positive for the 'pro-inflammatory' cytokines
       interleukin-1 alpha (IL-1 alpha) and tumour necrosis factor-alpha
       (TNF-alpha) were localized in the skin of infested mice, as detected at
       the mRNA level by in situ hybridization and at protein level by
       immunostaining with antibodies. These results suggest that an antigen
       was presented to infiltrating T lymphocytes which then became activated.
       This event may explain the cutaneous delayed-type hypersensitivity
       previously described in tick-infected BALB/c mice. Importantly, this
       cutaneous reaction was not sufficient to protect the mouse against tick
       re-infestation. Furthermore, ICAM-1 could mediate, at least in part, the
       extravasation of inflammatory cells into the skin of infested mice.
 DE    Animal  Antigens, Surface/*ANALYSIS  Cytokines/*BIOSYNTHESIS  *CD4-CD8
       Ratio  Female  Histocompatibility Antigens Class II/ANALYSIS
       Immunoenzyme Techniques  Intercellular Adhesion Molecule-1/ANALYSIS
       Interleukin-1/BIOSYNTHESIS  Mice  Mice, Inbred BALB C  Recurrence
       Skin/*IMMUNOLOGY  Support, Non-U.S. Gov't  Tick Infestations/*IMMUNOLOGY
       Tumor Necrosis Factor/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).