Document 0142
 DOCN  M9550142
 TI    Novel mechanism and factor for regulation by HIV-1 Tat.
 DT    9505
 AU    Zhou Q; Sharp PA; Center for Cancer Research, Massachusetts Institute
       of; Technology, Cambridge 02139.
 SO    EMBO J. 1995 Jan 16;14(2):321-8. Unique Identifier : AIDSLINE
       MED/95137012
 AB    Tat regulation of human immunodeficiency virus (HIV) transcription is
       unique because of its specificity for an RNA target, TAR, and its
       ability to increase the efficiency of elongation by polymerase. A
       reconstituted reaction that is Tat-specific and TAR-dependent for
       activation of HIV transcription has been used to identify and partially
       purify a cellular activity that is required for trans-activation by Tat,
       but not by other activators. In the reaction, Tat stimulates the
       efficiency of elongation by polymerase, whereas Sp1 and other DNA
       sequence-specific transcription factors activate the rate of initiation.
       Furthermore, while TATA binding protein (TBP)-associated factors (TAFs)
       in the TFIID complex are required for activation by transcription
       factors, they are dispensable for Tat function. Thus, Tat acts through a
       novel mechanism, which is mediated by a specific host cellular factor,
       to stimulate HIV-1 gene expression.
 DE    Fungal Proteins/METABOLISM  Gene Expression Regulation, Viral  Gene
       Products, tat/*PHYSIOLOGY  Hela Cells  Human  HIV-1/*GENETICS  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Trans-Activation (Genetics)
       Trans-Activators/METABOLISM  Transcription Factors/METABOLISM
       Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).