Document 0192
 DOCN  M9550192
 TI    Biological and genetic changes in ovine lentivirus strains following
       passage in isogeneic twin lambs.
 DT    9505
 AU    Woodward TM; Carlson JO; de la Concha-Bermejillo A; DeMartini JC;
       Department of Pathology, Colorado State University, College of;
       Veterinary Medicine and Biomedical Sciences, Fort Collins 80523.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Feb 1;8(2):124-33.
       Unique Identifier : AIDSLINE MED/95135990
 AB    Ovine lentivirus (OvLV) strains vary in cytopathogenicity, but the
       correlation, if any, between viral cytophenotypes and in vivo
       pathogenicity is poorly understood. To examine this issue and to
       evaluate changes in OvLV strains following passage in vivo, biological
       and genetic characteristics of OvLV isolates following in vivo passage
       were compared with those of the parent strain used for inoculation of
       two sets of isogeneic twin lambs. Plaque-purified OvLV strains with
       slow/low (84/28) and rapid/high (85/34) cell culture phenotypes were
       used for inoculation of the lambs. The phenotypes of the parent OvLV
       strains were compared with virus isolates from the four lambs by
       assaying virus replication and cytopathogenicity in goat synovial
       membrane cells. Viral population genetic differences in the env region
       were compared by polymerase chain reaction (PCR) amplification and
       denaturing gradient gel electrophoresis (DGGE) of the fragments. Virus
       isolates recovered from rapid/high virus-infected sheep were more lytic
       and developed syncytia earlier than viruses reisolated from sheep
       inoculated with the slow/low strain. Isolates from lambs infected with
       84/28 were more cytopathic in all assays than was their parent strain.
       Isolates from animals infected with 85/34 were more lytic and
       syncytiogenic than the parent strain, but responded similarly in
       replication assays. Although there were no consistent phenotypic
       differences between virus isolates recovered from sets of twins with
       markedly different degrees of lymphoid interstitial pneumonia (LIP), the
       DGGE band patterns of PCR amplified env fragments of the virus isolates
       from the twin lamb set with severe LIP, but not the set with slight LIP,
       were distinctly different from those of the parental viruses.
 DE    Animal  Base Sequence  Cytopathogenic Effect, Viral  Genes,
       env/*GENETICS  Molecular Sequence Data  Pneumonia, Progressive
       Interstitial, of Sheep/*VIROLOGY  Serial Passage  Sheep  Support, U.S.
       Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  Twin Studies  Twins,
       Monozygotic  *Variation (Genetics)  Virus Replication  Visna-Maedi
       Virus/GROWTH & DEVELOPMENT/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).