Document 0223
 DOCN  M9550223
 TI    Mother-to-child HIV-1 transmission: quantitative assessment of viral
       burden as a diagnostic tool and prognostic parameter in HIV-1-infected
       children.
 DT    9505
 AU    De Rossi A; Ometto L; Zanotto C; Salvatori F; Masiero S; Mammano F;
       Chieco-Bianchi L; Institute of Oncology, University of Padua, Italy.
 SO    Acta Paediatr Suppl. 1994 Aug;400:25-8. Unique Identifier : AIDSLINE
       MED/95135013
 AB    Polymerase chain reaction was performed in 251 infants born to
       HIV-1-seropositive mothers to diagnose HIV-1 infection. Assay
       specificity was invariably > 95%, regardless of age at testing, while
       sensitivity ranged from 15% in neonates (within 48 h of birth) to > 95%
       in infants over 1 month of age. Evaluation of viral burden in 43
       infected infants by means of quantitative DNA-PCR disclosed that the
       number of HIV-1 proviruses ranged from 5 to 947 per 100,000 peripheral
       blood mononuclear cells. Clinical follow-up demonstrated that a high
       viral burden was associated significantly with disease onset.
 DE    *Disease Transmission, Vertical  DNA, Viral/*ANALYSIS  Female  Human
       HIV Antibodies/BLOOD  HIV Antigens/BLOOD  HIV Core Protein p24/BLOOD
       HIV Envelope Protein gp120/BLOOD  HIV
       Infections/*DIAGNOSIS/IMMUNOLOGY/TRANSMISSION/VIROLOGY  *HIV-1  Infant
       Infant, Newborn  Leukocytes, Mononuclear/VIROLOGY  *Mothers  *Polymerase
       Chain Reaction  Prognosis  Sensitivity and Specificity  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).