Document 0251
 DOCN  M9550251
 TI    Isolation and identification of Encephalitozoon hellem from an Italian
       AIDS patient with disseminated microsporidiosis.
 DT    9505
 AU    Scaglia M; Sacchi L; Gatti S; Bernuzzi AM; Polver P de P; Piacentini I;
       Concia E; Croppo GP; da Silva AJ; Pieniazek NJ; et al; Laboratory of
       Clinical Parasitology, Pavia University-IRCCS San; Matteo, Italy.
 SO    APMIS. 1994 Nov;102(11):817-27. Unique Identifier : AIDSLINE
       MED/95134377
 AB    Microsporidia are primitive mitochondria-lacking spore-forming
       eukaryotic protozoa that infect a wide variety of animals and also
       humans. Of the five genera (Encephalitozoon, Enterocytozoon, Septata,
       Nosema and Pleistophora) that cause infections in humans, Enterocytozoon
       bieneusi, Septata intestinalis, and Encephalitozoon hellem are being
       increasingly identified in patients with acquired immunodeficiency
       syndrome (AIDS). E. bieneusi causes gastrointestinal disease, S.
       intestinalis causes gastrointestinal and disseminated disease, and E.
       hellem causes ocular as well as disseminated disease. We have
       established in continuous culture a strain of microsporidia isolated
       from the urine and throat washings of an Italian AIDS patient and
       identified it as Encephalitozoon hellem, based on its ultrastructural
       morphology, antigenic pattern, and polymerase chain reaction-amplified
       small subunit ribosomal RNA. We believe that this is the first time that
       a strain of microsporidia has been isolated from the throat washings of
       a patient with microsporidiosis.
 DE    Adult  Animal  AIDS-Related Opportunistic Infections/*PARASITOLOGY  Base
       Sequence  Cell Line  Cercopithecus aethiops  DNA Primers
       Encephalitozoon/GROWTH & DEVELOPMENT/*ISOLATION & PURIF/  ULTRASTRUCTURE
       Encephalitozoonosis/*COMPLICATIONS/PARASITOLOGY  Fluorescent Antibody
       Technique  Human  Immunoblotting  Italy  Microscopy, Electron
       Microscopy, Electron, Scanning  Molecular Sequence Data
       Pharynx/PARASITOLOGY  RNA, Ribosomal/GENETICS  Support, Non-U.S. Gov't
       Vero Cells  JOURNAL ARTICLE

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