Document 0468
 DOCN  M9550468
 TI    Alpha-lipoic acid increases intracellular glutathione in a human
       T-lymphocyte Jurkat cell line.
 DT    9505
 AU    Han D; Tritschler HJ; Packer L; Department of Molecular and Cell
       Biology, University of; California at Berkeley 94720-3200.
 SO    Biochem Biophys Res Commun. 1995 Feb 6;207(1):258-64. Unique Identifier
       : AIDSLINE MED/95160683
 AB    The addition of exogenous alpha-lipoic acid to cellular medium causes a
       rapid increase of intracellular unbound thiols in Jurkat cells, a human
       T-lymphocyte cell line. The rise of cellular thiols is a result of the
       cellular uptake and reduction of lipoic acid to dihydrolipoic acid and a
       rise in intracellular glutathione. Although the level of dihydrolipoic
       acid is 100-fold lower than glutathione, the cellular concentration of
       dihydrolipoic acid might be responsible for the modulation of total
       cellular thiol levels. Rises in glutathione correlate with the levels of
       intracellular dihydrolipoic acid (p < .01). This increase in glutathione
       is not the result of expression of new proteins like
       gamma-glutamylcysteine synthetase, since the rise in glutathione was not
       inhibited by cycloheximide, a protein synthesis inhibitor. Lipoic acid
       administration is therefore a potential therapeutic agent in an array of
       diseases with glutathione anomalies including HIV infection.
 DE    Cell Line  Chromatography, High Pressure Liquid  Dose-Response
       Relationship, Drug  Glutathione/*METABOLISM  Human  Kinetics
       T-Lymphocytes  Thioctic Acid/ANALOGS &
       DERIVATIVES/ANALYSIS/*PHARMACOLOGY  Time Factors  Tumor Cells, Cultured
       JOURNAL ARTICLE

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