Document 0472
 DOCN  M9550472
 TI    Early detection of antibodies to human immunodeficiency virus 1 by a
       third-generation enzyme immunoassay. A comparative study with the
       results of second-generation immunoassays and western blot.
 DT    9505
 AU    Barbe F; Klein M; Badonnel Y; Laboratoire de chimie biologique,
       maternite regionale de Nancy,; France.
 SO    Ann Biol Clin (Paris). 1994;52(5):341-5. Unique Identifier : AIDSLINE
       MED/95160327
 AB    The aim of the study was to examine the sensitivity and the specificity
       of a HIV-1/HIV-2 third-generation enzyme immunoassay, the Abbott
       recombinant HIV-1/HIV-2 third-generation enzyme immunoassay, which is
       reported to detect simultaneously IgG and IgM. Sensitivity was evaluated
       with sera from seropositive subjects and a series of samples from eight
       HIV-1 seroconverting subjects. Results were compared with Western blot,
       second-generation immunoassays (including Vidas HIV-1 + 2) and an HIV-1
       antigen assay. Specificity was studied with sera collected from a
       low-risk population and sera with false-reactive enzyme immunoassays
       results. In seven cases, the third-generation immunoassay detected
       HIV-antibodies several days earlier (range 4 to > or = 9 days) than the
       Western blot test according to the criteria proposed by the Association
       of State and Territorial Public Health Laboratory Directors, ie,
       positive with two of the three bands-gp160 or gp120, gp41, and p24. In
       the last case detection occurred at the same time as Western blot. The
       second-generation tests generally detected HIV-antibodies at the same
       time as Western blot. Antigaenemia was positive in the first samples
       tested in most cases, prior to or simultaneously with the Abbott
       third-generation test first reactivity, before the second generation
       tests and the Western blot test. In most cases, the disappearance of
       detectable HIV antigen was observed, and was concurrent with the
       development of the antibodies immune response. For our low-risk
       population, the current third-generation EIA test obtained a
       false-reactive rate of 0.26%. Our data indicate that the Abbott
       third-generation immunoassay is more sensitive than the Western blot
       test and the second-generation tests. The addition of a third-generation
       assay to the strategy for HIV-antibody screening may indeed be of
       interest and could make it possible to decrease the number of
       false-negative results.
 DE    Blotting, Western  Human  HIV Antibodies/*BLOOD  HIV Core Protein
       p24/IMMUNOLOGY  HIV Infections/DIAGNOSIS/*IMMUNOLOGY  HIV
       Seropositivity/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  IgG/BLOOD  IgM/BLOOD
       *Immunoenzyme Techniques  Sensitivity and Specificity  JOURNAL ARTICLE

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