Document 0477
 DOCN  M9550477
 TI    Ex vivo human placental transfer of human immunodeficiency virus-1 p24
       antigen.
 DT    9505
 AU    Bawdon RE; Gravell M; Roberts S; Hamilton R; Dax J; Sever J; Department
       of Obstetrics and Gynecology, University of Texas; Southwestern Medical
       Center, Dallas.
 SO    Am J Obstet Gynecol. 1995 Feb;172(2 Pt 1):530-2. Unique Identifier :
       AIDSLINE MED/95160057
 AB    OBJECTIVE: Our purpose was to determine whether human immunodeficiency
       virus-1 p24 antigen crosses the human placenta and, if so, to determine
       its clearance index relative to antipyrine. STUDY DESIGN: Eight term
       human placentas from uncomplicated vaginal or cesarean section
       deliveries were studied by ex vivo placental perfusion to determine the
       incidence and concentration required to obtain passage of p24 antigen
       into the fetal circulation. The concentration of p24 antigen was
       determined by antigen-capture enzyme immunoassay. RESULTS: The passage
       of p24 antigen into the fetal circulation was observed in three of five
       placentas studied when the p24 antigen concentration in the maternal
       circulation was 2942.8 +/- 401 pg/ml. When the p24 concentration in the
       maternal circulation was raised approximately fourfold to 14506 +/- 4124
       pg/ml, p24 antigen passed to the fetal circulation in two of three
       placentas and in three of three placentas in the closed perfusion
       system. CONCLUSIONS: p24 antigen crossed the human placenta to the fetal
       circulation in what appears to be a concentration-dependent manner.
 DE    Acquired Immunodeficiency Syndrome/TRANSMISSION
       Antipyrine/PHARMACOKINETICS  Disease Transmission, Horizontal  Human
       HIV Core Protein p24/*METABOLISM  HIV-1  In Vitro  Placenta/*METABOLISM
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).