Document 0488
 DOCN  M9550488
 TI    Activation of the interferon-inducible enzymes, 2',5'-oligoadenylate
       synthetase and PKR by human T-cell leukemia virus type I Rex-response
       element.
 DT    9505
 AU    Mordechai E; Kon N; Henderson EE; Suhadolnik RJ; Department of
       Biochemistry, Temple University School of Medicine,; Philadelphia,
       Pennsylvania 19140.
 SO    Virology. 1995 Feb 1;206(2):913-22. Unique Identifier : AIDSLINE
       MED/95159445
 AB    In vitro-synthesized human T-cell leukemia virus type 1 (HTLV-I) Rex
       response element (Rex-RE) activates the interferon-induced
       2',5'-oligoadenylate synthetase (2-50AS) in a dose-dependent manner. In
       addition, Rex-RE at 1 microgram/ml activates a second interferon-induced
       enzyme, p68 kinase (PKR); however, at 50 micrograms/ml, Rex-RE inhibits
       PKR activity. Poly(rl)-poly(rC) (10 micrograms/ml) dissociates the
       ribonucleoprotein complexes, Rex-RE/2-5OAS, or Rex-RE/PKR, whereas
       poly(rC) (100 micrograms/ml) does not, indicating the presence of high
       affinity interactions between Rex-RE and these two enzymes. To further
       characterize the interaction of Rex-RE with 2-5OAS and PKR, [32P]Rex-RE
       was uv-cross-linked to 2-5OAS and PKR present in interferon-treated HeLa
       cell extracts. The affinity of Rex-RE to highly purified 40-kDa human
       recombinant 2-5OAS was determined to be Kd = 4.7 nM. The relevance of
       these results to the pathogenesis of HTLV-I-associated adult T-cell
       leukemia/lymphoma is discussed.
 DE    Cross-Linking Reagents  Dose-Response Relationship, Drug
       Endoribonucleases  Enzyme Activation  Enzyme Induction  Hela Cells
       Human  HTLV-I/*PHYSIOLOGY  Interferon-alpha/*PHARMACOLOGY  Kinetics
       Poly C/PHARMACOLOGY  Poly I-C/PHARMACOLOGY  Protein-Serine-Threonine
       Kinases/*BIOSYNTHESIS/ISOLATION & PURIF  Recombinant
       Proteins/BIOSYNTHESIS/ISOLATION & PURIF  RNA, Viral/*METABOLISM
       Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       Ultraviolet Rays  2',5'-Oligoadenylate
       Synthetase/*BIOSYNTHESIS/ISOLATION & PURIF  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).