Document 0493
 DOCN  M9550493
 TI    Differences in feline immunodeficiency virus host cell range correlate
       with envelope fusogenic properties.
 DT    9505
 AU    Pancino G; Castelot S; Sonigo P; Genetique des Virus (ICGM-CNRS UPR
       0415) Institut Cochin de; Genetique Moleculaire, Paris, France.
 SO    Virology. 1995 Feb 1;206(2):796-806. Unique Identifier : AIDSLINE
       MED/95159433
 AB    Feline immunodeficiency virus (FIV) establishes persistent infections in
       cats inducing an acquired immunodeficiency syndrome. Differences in cell
       tropism have been observed among isolates of FIV (T. R. Phillips et al.,
       J. Virol. 64, 4605-4613, 1990). The progeny of the infectious molecular
       clone of FIV p34TF10 was able to productively infect a feline fibroblast
       cell line, Crandell feline kidney cell, (CrFK), while the progeny of the
       molecular clone pPPR was not. However, pPPR, after transfection of CrFK
       cells, did produce virions which were able to productively infect feline
       lymphocytes. To analyze the mechanisms responsible for such differences
       in tropism and particularly the role of the envelope glycoproteins
       (Env), Env expression vectors were constructed by deletion of gag and
       pol genes from 34TF10 and PPR proviral clones. Env expression and
       function were studied by using a syncytium-formation assay and a
       quantitative ELISA. After transfection of CrFK, both 34TF10 and PPR Env
       precursors were correctly processed and Env surface glycoprotein, gp100,
       was released in culture supernatants. However, the Env of 34TF10 caused
       a dramatic syncytial effect in CrFK cells, while PPR Env did not induce
       any syncytium formation. The Env of 34TF10 placed under the control of
       the long terminal repeat of PPR maintained its ability to induce CrFK
       fusion. These results suggest that the inability of FIV PPR to infect
       CrFK fibroblasts is related to a restriction of virus entry mediated by
       the viral envelope.
 DE    Animal  Base Sequence  Cats  Cell Line  Cloning, Molecular  Comparative
       Study  DNA Primers  Enzyme-Linked Immunosorbent Assay  Gene Deletion
       Gene Products, env/*BIOSYNTHESIS/GENETICS  Genes, gag  Genes, pol  Giant
       Cells  Immunodeficiency Virus, Feline/GENETICS/*PHYSIOLOGY  Kidney
       Kinetics  Membrane Fusion  Molecular Sequence Data  Plasmids  Polymerase
       Chain Reaction  Support, Non-U.S. Gov't  Time Factors  Transfection
       Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).