Document 0494
 DOCN  M9550494
 TI    Functional similarities between HIV-1 Tat and DNA sequence-specific
       transcriptional activators.
 DT    9505
 AU    Madore SJ; Cullen BR; Howard Hughes Medical Institute, Duke University
       Medical Center,; Durham, North Carolina 27710.
 SO    Virology. 1995 Feb 1;206(2):1150-4. Unique Identifier : AIDSLINE
       MED/95159430
 AB    The Tat regulatory protein encoded by human immunodeficiency virus type
       1 (HIV-1) induces high levels of transcription from the viral long
       terminal repeat (LTR) promoter element after interacting with a promoter
       proximal RNA target sequence. In the wild-type HIV-1 LTR, this
       activation is facilitated by the synergistic interaction of Tat with the
       NF-kappa B and, particularly, SP1 regulatory proteins that bind to DNA
       sequences within the LTR promoter element. Using a synthetic Tat
       responsive indicator construct, we here demonstrate that NF-kappa B and
       SP1 are not uniquely or even unusually competent to synergize with HIV-1
       Tat. Instead, these proteins can be functionally replaced by several,
       but not all, of the heterologous cellular and viral transcriptional
       activators tested. Tat therefore shares the ability to functionally
       synergize with a range of transcriptional activators, which is
       characteristic of DNA-sequence-specific regulatory proteins.
 DE    Animal  Binding Sites  Cell Line  Cercopithecus aethiops  Comparative
       Study  Cytomegalovirus/GENETICS/METABOLISM  DNA, Viral/*METABOLISM  Gene
       Products, tat/*METABOLISM  Human  *HIV Long Terminal Repeat
       HIV-1/GENETICS/*METABOLISM  Kidney  NF-kappa B/METABOLISM  Plasmids
       Promoter Regions (Genetics)  Recombinant Fusion Proteins/METABOLISM
       Trans-Activation (Genetics)  Trans-Activators/*METABOLISM  Transcription
       Factor, Sp1/METABOLISM  Transcription, Genetic  Transfection  JOURNAL
       ARTICLE

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