Document 0495
 DOCN  M9550495
 TI    Cellular tropism of human T-cell leukemia virus type II is enlarged to B
       lymphocytes in patients with high proviral load.
 DT    9505
 AU    Casoli C; Cimarelli A; Bertazzoni U; Istituto di Patologia Speciale
       Medica, Universita di Parma,; Italy.
 SO    Virology. 1995 Feb 1;206(2):1126-8. Unique Identifier : AIDSLINE
       MED/95159425
 AB    To establish the in vivo cellular tropism of human T-cell leukemia virus
       type II (HTLV-II) in peripheral blood, subpopulations of mononuclear
       cells isolated from patients with a history of drug abuse and with high
       proviral load were analyzed by polymerase chain reaction for the
       presence of the proviral sequences. After purification of cellular
       subsets by immunomagnetic fractionation of blood cells of an infected
       patient, HTLV-II DNA was detected in CD4+ and CD8+ T-cells as well as in
       CD19+ B-cells. A positive PCR signal was obtained for purified B-cells
       also at limiting dilutions. This observation was confirmed by purifying
       the B-cell fraction by a two-step immunomagnetic procedure from the
       peripheral blood of another patient with very high HTLV-II copy number
       and quantifying the B-cell proviral load by means of competitive PCR. A
       proviral copy number of 90/100 B-cells was found, demonstrating that the
       great majority of these cells were infected by HTLV-II in this subject.
       The results indicate that HTLV-II has a broad host range in some
       infected individuals, showing an enlargement of cellular tropism to B
       lymphocytes and suggesting that this expression is associated with an
       increase in proviral load.
 DE    Antibodies, Monoclonal  Antigens, CD/ANALYSIS  Antigens,
       Differentiation, B-Lymphocyte/ANALYSIS  B-Lymphocytes/*VIROLOGY  CD4-CD8
       Ratio  Human  HTLV-II/ISOLATION & PURIF/*PHYSIOLOGY  HTLV-II
       Infections/IMMUNOLOGY/*VIROLOGY  Polymerase Chain Reaction  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).