Document 0499
 DOCN  M9550499
 TI    Porcine peripheral blood CD4+/CD8+ dual expressing T-cells.
 DT    9505
 AU    Pescovitz MD; Sakopoulos AG; Gaddy JA; Husmann RJ; Zuckermann FA;
       Department of Surgery, Indiana University, Indianapolis 46202.
 SO    Vet Immunol Immunopathol. 1994 Oct;43(1-3):53-62. Unique Identifier :
       AIDSLINE MED/95159401
 AB    The porcine T-cell population is unique in that there is a large
       percentage of CD+CD8+ dual expressing peripheral T-cells. This paper
       reviews the data available on these porcine T-cells and compares them to
       the much rarer dual expressing T-cells in humans. The percent of dual
       expressing cells increases with activation in in vitro culture with
       various antigens including pseudorabies virus. The percent of resting
       dual expressing cells also increases with the age of the pig.
       Flow-cytometric-sorted dual expressing cells responded in culture to the
       super antigen staphylococcal enterotoxin B. Selected CD4+CD8- cells
       cultured in vitro developed expression of CD8 and maintained the dual
       expressing phenotype for the 12 weeks of culture. Dual expressing cells
       freshly prepared from porcine blood did not express the IL-2 receptor as
       demonstrated by their failure to bind FITC-IL-2 and an anti-porcine IL-2
       receptor monoclonal antibody. In response to activation with phorbol
       myristic acetate, CD4, but not CD8, was down regulated on the dual
       expressing T-cells. In summary, porcine dual expressing T-cells
       constitute a substantial percentage of the porcine peripheral T-cell
       pool. These cells appear to contain the majority of the memory T-cell
       with their frequency increasing with blood donor age and in vitro
       culture. Although the receptor specificity is not known, they have a
       functional receptor. Finally, the function of the two accessory
       molecules CD4 and CD8 in these cells is not known, but their regulation
       is distinct, thereby suggesting no equivalent roles in immune function.
 DE    Animal  Antigens, CD4/*IMMUNOLOGY  Antigens, CD8/*IMMUNOLOGY
       Comparative Study  CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Flow Cytometry  Human  Receptors, Antigen,
       T-Cell/IMMUNOLOGY  Swine/*IMMUNOLOGY  JOURNAL ARTICLE  REVIEW  REVIEW,
       TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).