Document 0517
 DOCN  M9550517
 TI    [Possibility for detecting HIV-1 in bone transplant by PCR using the
       HIV-1 microtiter plate assay]
 DT    9505
 AU    Roder W; Kruse M; Runkel M; Muller WE; Isemer FE; Abteilung fur
       Allgemein- und Unfallchirurgie, St.; Josefs-Hospital Wiesbaden.
 SO    Unfallchirurg. 1994 Dec;97(12):629-32. Unique Identifier : AIDSLINE
       MED/95158886
 AB    It is known that HIV can be transmitted by allogenous bone
       transplantation. Hitherto neither chemical nor physical methods have
       existed to allow reliable disinfection and sterilization of bone
       specimens without reducing osteogenetic potency. Only demonstration or
       exclusion of the presence of HIV-1 in a bone specimen guarantees that
       infection will not occur. The method now presented for HIV detection is
       based on a polymerase chain reaction (PCR). This HIV microtiter-plate
       assay combines amplification of DNA molecules with a staining reaction.
       In cultures containing HIV-infected cells definite detection of viruses
       was possible when 50-100 cells per specimen were infected. Examination
       of 137 HIV-negative and 25 HIV-positive bone specimens showed
       sensitivity of 96% and specificity of 97.8% for the test. In subsequent
       studies, after drying on filter paper viral DNA was again demonstrable
       by the PCR. This means safe handling and uncomplicated transportation of
       non-infectious specimens to a central analysis laboratory are possible.
       This HIV test offers the possibility of quick and safe demonstration
       that specimens are free of HIV and is therefore likely to enhance the
       safety of bone transplantation considerably.
 DE    Bone and Bones/*VIROLOGY  *Bone Transplantation  English Abstract  Human
       HIV Infections/PREVENTION & CONTROL/*TRANSMISSION/VIROLOGY
       HIV-1/*ISOLATION & PURIF  Polymerase Chain Reaction/*METHODS  Risk
       Factors  Transplantation, Homologous  JOURNAL ARTICLE

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