Document 0559
 DOCN  M9550559
 TI    Synthetic approaches to continuous assays of retroviral proteases.
 DT    9505
 AU    Krafft GA; Wang GT; Department of Structural Biology, Drug Design and
       Delivery,; Abbott Laboratories, Abbott Park, Illinois 60064.
 SO    Methods Enzymol. 1994;241:70-86. Unique Identifier : AIDSLINE
       MED/95157325
 AB    This chapter has described a number of approaches for continuous assay
       of retroviral proteases using either chromogenic or fluorogenic
       synthetic substrates. The significant progress in this area has been
       catalyzed by the intense interest in HIV protease as a therapeutic
       target, but these versatile methods will be used widely in future for
       studies of many other proteases.
 DE    Amino Acid Sequence  Aspartic Proteinases/*ANALYSIS/METABOLISM
       Chromogenic Compounds/METABOLISM  Colorimetry/METHODS  Fluorescent Dyes
       Fluorometry/METHODS  Hydrolysis  HIV Protease/ANALYSIS/METABOLISM
       Molecular Sequence Data  Peptide Fragments/ANALYSIS  Peptides/CHEMICAL
       SYNTHESIS/METABOLISM  Retroviridae/*ENZYMOLOGY  Retroviridae
       Proteins/*ANALYSIS/METABOLISM  Sensitivity and Specificity  Substrate
       Specificity  Time Factors  JOURNAL ARTICLE  REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).